所屬科目：研究所、轉學考(插大)◆生物化學
1. Which below wavelength is commonly used to detect aromatic amino acids? (A)230nm (B)260nm (C)280nm (D)320nm (E)488nm
2. Which a-carbon bonded group in cysteine functions as a nucleophile? (A)-H group (B)Amino-group (C)Carboxyl-group (D)SH-group (E)-OH group
3. Which below amino acid cannot be modified by phosphorylation? (A)Serine (B)Tyrosine (C)Histidine (D)Threonine (E)Alanine
4. Which below method cannot be used to disrupt cells for protein purification? (A)Cell lysis (B)French press (C)Enzyme digestion (D)Grinding (E)Centrifugation
5. Which of the following enzymes possesses both RNA-dependent DNA polymerase and DNA-dependent DNA polymerase activities? (A)DNA polymerase I (B)DNA polymerase II (C)DNA polymerase III (D)Klenow fragment (E)Reverse transcriptase
6. Which of the following polymerase synthesizes 5S rRNA? (A)RNA polymerase I (B)RNA polymerase II (C)RNA polymerase III (D)Telomerase (E)Reverse transcriptase
7. Which of the following enzymes is a ribozyme? (A)RNase A (B)RNase D (C)RNase H (D)RNase P (E)RNase III
8. Which of the following factors is critical for the maintenance of lysogenic state of lambda phage? (A)Cro protein (B)Lambda repressor (C)N protein (D)Q protein (E)Sigma factor
9. The expression of kappa light chain of antibodies is controlled by which of the following gene regulations? (A)Gene deletion (B)Gene translocation (C)Gene amplification (D)Gene conjugation (E)Gene insertion
10. Which of the following templates is NOT a substrate for polymerase chain reaction (PCR)? (A)Double strand DNA (B)Single strand DNA (C)Circular form of DNA (D)mRNA (E)Plasmid DNA.
11. At replication forks in E. coli: (A)DNA helicases make endonucleolytic cuts in DNA. (B)DNA primers are degraded by exonucleases. (C)DNA topoisomerases make endonucleolytic cuts in DNA. (D)RNA primers are removed by primase. (E)RNA primers are synthesized by primase.
12. The function of the eukaryotic DNA replication factor PCNA (proliferating cell nuclear antigen) is similar to that of the B-subunit of bacterial DNA polymerase III in that it: (A)facilitates replication of telomeres. (B)increase the processivity of replication. (C)has a 3' → 5' proofreading activity. (D)increases the speed but not the processivity of the replication complex. (E)participates in DNA repair.
13. Which of the following statements regarding plasmid cloning vectors is correct? (A)Circular plasmids do not require an origin of replication to be propagated in E. coli. (B)Foreign DNA fragments up to 45,000 base pairs can be cloned in a typical plasmid. (C)Plasmids do not need to contain genes that confer resistance to antibiotics. (D)Plasmid vectors must carry promoters for inserted gene fragments. (E)The copy number of plasmids may vary from a few to several hundred.
14. In homologous recombination in E. coli, the protein that assembles into long, helical filaments that coat a region of DNA is: (A)RecA protein. (B)RecBCD enzyme. (C)DNA methylase. (D)DNA polymerase. (E)Histone.
15. Apolipoprotein AI (Apo-AI) deficiency is associated with (A)low plasma total cholesterol (B)high plasma total cholesterol (C)high plasma triacylglycerol (D)low plasma LDL-cholesterol (E)low plasma HDL-cholesterol
16. Which of the following is not required for synthesis of arachidonic acid (C20:4, n-6) from linoleic acid (C18:2, n-6)? (A)Δ4-desaturase (B)Δ5-desaturase (C)Δ6-desaturase (D)Elongase (E)Malonyl-CoA
17. Which enzyme is the key enzyme for bile acid biosynthesis? (A)Acetyl-CoA carboxylase (B)7α-hydroxylase (C)HMG-CoA reductase (D)HMG-CoA lyase (E)Acyl-CoA synthetase
18. Which of the following is responsible for the generation of LDL from VLDL? (A)HMG-CoA reductase (B)HMG-CoA lyase (C)Hormone sensitive lipase (D)Lipoprotein lipase (E)Pancreatic lipase
19. What molecule is it that provides 8 electrons for nitrogen fixation? (A)Glucose (B)Oxaloacetate (C)Pyruvate (D)Acetyl-coA (E)ATP
20. What enzyme is it that mediates nitrogen entry into higher organisms? (A)Glutamine synthetase (B)Glutamate-pyruvate transaminase (C)Carbamoyl phosphate synthetase I (D)Arginase (E)N-acetylglutamate synthase
21. Which transporting protein is it that works via secondary active transport mechanism? (A)Ionophore (B)Ion channel (C)Uniporter (D)Symporter (E)Pump
22. Which of the phospholipid is more abundant in the outer membrane of a lipid bilayer? (A)Phosphatidylserine (B)Phosphatidylcholine (C)Phosphatidylinositol (D)Phosphatidylethanolamine (E)Phosphatidic acid
23. What following component cannot be a cofactor for a dehydrogenase? (A) (B) (C)FAD (D)Copper (E)FMN
24. How many following enzyme(s) in the list can generate a product of glucose? (i) Phosphorylase, (ii) Phosphoglucomutase, (iii) Glucose 6-phosphatase, (iv) Fructose 2,6-bisphosphatase, (v) Debranching enzyme. (A)1 (B)2 (C)3 (D)4 (E)5
25. Which following component can directly inhibit phosphofructokinase? (A)Fructose 2,6-bisphosphate (B)Fructose 6-phosphate (C)Citrate (D)Malate (E)Alanine.
26. What amino acid is responsible for the generation of proline in human cells? (A)Serine (B)Lysine (C)Phenylalanine (D)Asparagine (E)Glutamate.
27. What must happen in order to prevent overstimulation by a hormone? (A)Hormones must be degraded. (B)G-proteins must be recycled and then degraded. (C)Receptors must be blocked from continuing to activate G-proteins. (D)Receptors must dimerize. (E)All of above.
28. Which of the following hormones termed the "flight-or-fight" hormone is secreted by the adrenal medulla? (A)Epinephrine (B)Oxytocin (C)Insulin (D)Glucagon (E)Somatostatin
29. Which hormone is secreted by alpha cells in the pancreas in response to low blood glucose levels? (A)Insulin (B)Glucagon (C)Estradiol (D)Epinephrine (E)Somatostatin
30. In liver cells, the expression of genes encoding gluconeogenic enzymes such as phosphoenolpyruvate carboxykinase is induced in response to which of the following molecules? (A)cGMP (B)Insulin (C)ATP (D)cAMP (E)Cholesterol
31. Which of the following amino acids is required for conversion of inosine monophosphate (IMP) to adenylosuccinate en route to adenosine monophosphate (AMP)? (A)Aspartate (B)Glutamine (C)Glycine (D)Glutamate (E)Asparagine.
32. Which of the following descriptions about small noncoding regulatory RNAs is RIGHT ? (A)siRNAs are frequently used to knock-out specific protein levels (B)miRNAs are double stranded (C)miRNAs can cause translational repression, mRNA destabilization and mRNA degradation (D)All are right (E)All are wrong.
33. Which of the following molecules is an intermediate in the catabolism of adenine and guanine? (A)Hypoxanthine (B)Xanthine (C)Uric acid (D)All are right (E)All are wrong.
34. In the urea cycle, which of the following enzymes is in the matrix of liver mitochondria to catalyze reactions? (A)Arginase (B)Argininosuccinate synthase (C)Argininosuccinate lyase (D)Carbamoyl phosphate synthase I (E)All of them.
35. A 36-year-old man is diagnosed with Arsenic poisoning, that inhibits which of the following enzymes of TCA cycle? (A)Citrate synthase (B)Malate dehydrogenase (C)Aconitase (D)a-ketoglutarate dehydrogenase (E)None of the above
36. During strenuous exercise, the most important reaction involved in the reoxidation of NADH is: (A)dihydroxyacetone phosphate → glycerol 3-phosphate (B)glucose 6-phosphate → fructose 6-phosphate (C)isocitrate → a-ketoglutarate (D)pyruvate→ lactate (E)None of the above
37. Which of the following statements about the TCA cycle is correct? (A)Oxygen is used to oxidize the acetyl group carbons of acetyl-CoA in the TCA cycle. (B)Three molecules of NADH and one molecule of FADH2 are produced in one turn of the TCA cycle. (C)Oxygen is not used in the TCA cycle, so the cycle can occur in anaerobic conditions. (D)The TCA cycle produces the water that is formed during the complete oxidation of glucose. (E)None of the above
38. In what form does the product of glycolysis enter the TCA cycle? (A)Pyruvate (B)Acetyl-CoA (C)NADH (D)Glucose (E)None of the above
39. In prokaryotic translation initiation, start codon forms base pairs with which molecule : (A)16S rRNA. (B)Shine-Dalgarno sequence. (C)IF-2-GTP. (D)fMet-tRNA Met (E)23S rRNA.
40. For targeting proteins to endomembrane system, signal sequences are recognized by: (A)Proteasome. (B)Importin. (C)Ribosome. (D)Ion channels. (E)SRP.
41. What kind of mutation at protein-coding regions of genome can cause a frame shift in translation: (A)A single nucleotide insertion. (B)A nonsense mutation. (C)A missense mutation. (D)A silent mutation. (E)Initiation failure.
42. Which molecule can promote translocation in translation elongation? (A)EF-Ts. (B)NES. (C)EF-G. (D)EF-Tu. (E)eIF-4.
43. The amino acid glycine is known as an α-helix breaker. Thus, a glycine-rich sequence should NOT form an α-helix, why? (A)Glycine cannot form the typical i→ i+4 hydrogen bond. (B)The side-chain of glycine is too large. (C)Glycine is structurally the most flexible amino acid. (D)The side-chain of glycine is negatively charged. (E)Glycine is too hydrophobic
44. Enzymes increase reaction rates by.... (A)binding tightly to substrate (B)increasing the ΔG° of a reaction (C)decreasing the ΔG° of a reaction (D)increasing the activation energy of a reaction (E)decreasing the activation energy of a reaction
45. Which statement regarding protein structure is NOT correct? (A)The tertiary structure of a protein is specified by its primary structure. (B)The tertiary structures of most proteins are heat-resistant. (C)The formation of protein tertiary structure can be assisted by chaperones. (D)The majority of protein tertiary structures are stabilized by non-covalent interactions. (E)An α-helix may undergo conformational change to become a β-sheet.
46. Lyases are enzymes capable of catalyzing.... (A)breakage of chemical bonds (B)hydrolysis of chemical bonds (C)phosphorylation reaction (D)the conversion of ATP into ADP (E)isomerization of chemical bonds
47. Which enzyme is involved in cis Golgi oligosaccharides processing (A)N-acetyl glucosaminyltransferase I (B)N-acetyl glucosaminyltransferase II (C)Golgi apparatus alpha mannosidase (D)Oligosaccharide:protein transferase (E)Fucosyltransferase
48. Which integrin can bind to I-CAM involved in leukocyte trans endothelial cell layer during the inflammation. (A)αLβ2 (B)α2β1 (C)α3β1 (D)α5β1 (E)ανβ3
49. The following glycosaminoglycans which one is without protein core (A)Heparan sulfate (B)Dermatan sulfate (C)Heparin (D)Chondroitin sulfate (E)Keratan sulfate
50. Which description about cell death is not right (A)Basement membrane is required for cell survival call anchorage-independent growth (B)Apoptosis is the only one type of cell death (C)Cytochrome C releasing is critical for caspase-3 activation (D)Caspase-3 activation need the proteolytic cleave procaspase-3 (E)（A）and （B）are not right
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(B)
(C)FAD (D)Copper (E)FMN