113年 - 113 國立成功大學_碩士班招生考試_藥理學研究所 :分子生物學#126004

1. How many open reading frames can be possibly found in a double-stranded DNA? (A) 1 (B) 2 (C) 3 (D) 4 (E) 5 (F) 6

2. Which of the following labeling nucleotide will be used to study transcription initiation? (A) a-labeled NTP (B) B-labeled NTP (C) y-labeled NTP

3. Which of the following statements is   TRUE  ? (A) All genes can be only transcribed once during a cell cycle. (B) Genes can be overlapped and encode proteins with different amino acid sequences. (C) RNA transcript can be used only once to translate a protein. (D) Polyadenylation sequence can be found at the 3' of the gene sequence.

4. What is   NOT TRUE   descriptions for promoter and σ factor in E.coli? (A) Base substitution at -35 box will decrease promoter activity of E. coli. (B) Base deletion or insertion between -35 box and -10 box will decrease the promoter activity. (C) -10 box is the transcription start site for RNA transcript. (D) The function of -10 box is similar to that of TATA box of eukaryotic RNAPII promoter.

5. Which eukaryotic RNA polymerase locates in nucleolus? (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III

6. Which eukaryotic RNA polymerase is most sensitive to a-amanitin? (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III

7. Which is the assembling factor of subunits in prokaryotic RNA polymerase? (A) α (B) β (C) β' (D) σ

8. Which is the specificity factor of subunits in prokaryotic RNA polymerase? (A) α (B) β (C) β' (D) σ

9. Which of the following motifs DO NOT contain DNA-binding activity? (A) helix-turn-helix motif. (B) basic helix-loop-helix motif. (C) leucine zipper motif. (D) zinc finger motif.

10. Which of the following histone is   NOT   included in core histone? (A) H1 (B) H2A (C) H2B (D) H3 (E) H4

11. Which of the following is not true concerning eukaryotic mRNA processing? (A) addition of a 3' poly-A tail (B) addition of a 5' cap (C) intron splicing (D) occurs in the nucleus (E) occurs in the cytoplasm

12. The first two bases and the last two bases in the splicing signal consensus sequence are (A) GT-AG. (B) GU-AG. (C) CU-AG. (E) GT-TG. (D) GU-AC.

13. Which of the following is the name for the Yeast 40S particle where mRNA splicing takes place? (A) ribosome (B) nucleolus (C) spliceosome (D) hnRNA (E) R-loop

14. "Snurps" are composed of (A) RNA. (B) protein. (C) DNA. (D) RNA and protein. (E) DNA and RNA.

15. Which of the following snRNP is mismatched with its function? (A) U1: base pairs with 5' splice site of mRNA. (B) U2: base pairs with the conserved sequence at splicing branch point. (C) U4: base pairs with 3' splice site of mRNA. (D) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon. (E) U6: base pairs with 5' end of the intron.

16. The catalytic center of the spliceosome appears to include (A) Mg2+.(B) U2 and U6 snRNP. (C) the branch point region of the intron. (D) Mg2+ and the branch point region of the intron. (E) Mg2+, U2, and U6 snRNP, and the branch point region of the intron.

17. Please put the following steps of Cap synthesis in the correct order. 　

1: N7 of the capping guanine is methylated. 　

2: The terminal phosphate is removed from the pre-mRNA. 　

3: A capping GMP is added to the pre-mRNA. 　

4: The 2'-O-methyl group of the penultimate nucleotide is methylated. (A) 1, 2, 3, 4 (B) 1, 4, 3, 2 (C) 2, 4, 1, 3 (D) 2, 3, 1, 4 (E) 4, 3, 2, 1

18.(A) protects the mRNA from degradation (B) enhances translatability of the mRNA (C) enhances transport of the mRNA to the cytoplasm (D) enhances splicing of the mRNA (E) helps regulate expression of the mRNA

二、問答題:

1. Please describe   in detail   the essential components involved in protein synthesis and processes of protein translation in E. coli. 

2. Please describe   in detail   the mechanism of DNA replication in E.coli. 

(A) Nonsense mutation 

(B) Okazaki fragment

(C) Rolling circle DNA replication

(D) Nucleotide excision repair

(E) Retrotransposons