複選題

19. Regarding to the statements of the onco-immunology (IO), which are CORRECT?
(A) PD1/PDL1 is the immune checkpoint pathway
(B) Antibody to stimulate the PD1 has shown the therapeutic benefit for cancer patients
(C) Activation or up-regulation of PDL1 in tumor cells could inhibit the T-cell activation
(D) Lower level of PDL1 in tumor tissues might show the better outcome of patients with cancer immunotherapy (e.g., Pembrolizumab)
(E) The adverse effects of the cancer immunotherapies include interstitial pneumonitis

複選題15. Regarding to the nuclear receptor coactivators which statement(s) is (are) CORRECT? (A) The coactivator might contain the histone acetyl transferase (HAT) domain (B) The coactivator may contain the RNA binding domain (C) The mechanisms and activity could be the tissue-specificity to hormone-responses. (D) The coactivators are unique and specific to a specific nuclear receptor. (E) The co-activator might contribute to the regulation of gene expression.

複選題16. Regarding to the applications of fluorescence labbeled monoclonal antibody which statement(s) is (are) CORRECT? (A) To isolate sub-population of cells via flow cytometry  (B) To identify specific RNA expression via real time Q-PCR (C) To identify specific protein for immunofluorescence (IF) image. (D) To quantify the protein level via Western blotting (E) To identify a specific DNA adduct

17. What are the paired colors to show the colocalization of two fluorescent probes or two pseudo- colors in a specimen? (A) Alexa Fluor 488 and Cy3: orange and yellow (B) eGFR and DsRed: Blue (C) Blue and Red: Magenta (D) Blue and Green: Cyan (E) Green and Red: Yellow

複選題18. Regarding to the high throughput techniques and the "Omics", which pairs are CORRECT? (A) RNA-seq: Transcriptomics (B) Bisulfite sequencing: Epigenetics (C) Image-based high content analysis (HCA): Cytomics (D) LC-MS/MS: Proteomics (E) Q-PCR: Phosphoproteomics

複選題20. Which is (are) the RNA virus (has RNA as its genetic material)? (A) Human Papillomavirus (B) Influenza B virus (C) Severe Acute Respiratory Syndrome Coronavirus 2 (D) Hepatitis B virus (E) Human Immunodeficiency virus

複選題21. Which abbreviation of the following amino acids are CORRECT? (A) Y=Tyrosine (B) Q= Glutamic acid (C) W=Tryptophan (D) E= Aspartic acid (E) K= Lysine 

複選題22. Monoclonal antibody (mAb) is a powerful research tool and a drug for treating diseases. Regarding mAb which statements are NOT CORRECT? (A) mAb can be used to kill tumor cells or to block the binding of viruses or bacteria to host cells (B) The development and production of mAb is much cheaper than small molecule inhibitor drugs (C) Hybridoma technology is still the only way to generate mAbs (D) mAbs for immune checkpoint blockade such as CTLA-4 and PD-1 are successfully used for treating autoimmune diseases (E) mAbs can be used to target molecules located on the cell surface and inside the cells

複選題23. Mitochondria are the power plant of the cells that produce ATP for maintain cell homeostasis. Other than bioenergy what else do mitochondria do in the cells. (A) Regulate signal transduction (B) Regulate apoptosis (C) Regulate the functions of immune cells (D) Regulate reactive oxygen species (E) Regulate metabolism

24. CRISPR-Cas9 is a breakthrough of technology for genome editing. Which statements regarding CRISPR-Cas9 are NOT CORRECT? (A) Cas9 is an RNAse originally identified in bacteria (B) CRISPR sequences can be served as a guide to recognize and cleave specific strands of DNA (C) The mechanism of CRISPR-Cas9-meidated genome editing is working through dsDNA break and induced repair system in the host cells (D) CRISPR-Cas9 can only be used to generate knockout mice but not knockin mice (E) CRISPR-Cas9 can target multiple genes at a time

複選題25. Regarding reverse transcriptase, which statements are NOT CORRECT? (A) It has DNA-dependent RNA polymerase activity (B) It has RNA-dependent DNA polymerase activity (C) It is an important enzyme for doing polymerase chain reaction (PCR) (D) It has no proof-reading activity and therefore is error-prone (E) It was originally identified in Adenovirus