試題詳解

複選題
21.Which of the following conditions are required to assure production of a protein when a plasmid is being designed to express a eukaryotic protein in a bacterium?
(A) Introns must be deleted from the gene.
(B) The cloning site must include an RNA polymerase promoter.
(C) The mRNA product must contain a ribosome-binding site.
(D) The poly-A tail must be added to the 3' end of the gene.
(E) Differences in the genetic code between eukaryotes and prokaryotes must be accommodated.