試題詳解

29. Below is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Which of the following statements is/are FALSE?

(A) If a recombinant plasmid were obtained inserting DNA into the BamHI site, the recombinant plasmid would be resistant to the antibiotic tetracycline.
(B) If a recombinant plasmid were obtained by inserting DNA into the EcoRV site, the plasmid will be resistant to ampicillin.
(C) If the region between Ndel and PstI were deleted, the resulting plasmid would work well for cloning.
(D) If a recombinant plasmid was obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline.
(E) If the plasmid were completely digested with the three restriction enzymes BamHI, Ndel and PstI, the resulting fragments would be 1920, 1312, 754, and 375 base pairs long.