複選題

3. Which of the following methods can be used for protein sequencing?
(A) Mass spectrometry.
(B) Sanger sequencing.
(C) Two-dimensional (2D) gel electrophoresis.
(D) Next generation sequencing (NGS).

1899 下列何者不是CSD圖製作之主要操作步驟？(A)以建築圖為背景，以CAD套繪機電、給排水等設計資料，並以顏色設層(Layer)編排完整 (B)依功能檢視設計資料，將各層相互重疊部分逐一檢討，並將產生界面問題部分提出協調，並排除所有衝突點 (C)依預留管件之功能需求，檢討留設於結構體中之管件 大小及高程　(D)當CSD完成調整與整合，無衝突疑慮，依CSD內所有系統管線、設備需求，開始製作SEM圖。

1900 下列敘述何者為是(A)整合CSD過程中，以管徑大小為第一考量、功能為第二考量 (B)CSD圖主要目的是提供土建廠商於施工過程中將機電系統廠商所需預埋套管、預留開口及設備混凝土基座等，套合成圖 (C) CSD繪圖過程中，機電系統方面，則以弱電系統為第一優先考量 (D)施工時除了依CSD及 SEM所檢討出之相關圖面施工外，工地仍有許多界面點需檢討，可藉由施工界面協調會將現場發生之界面問題加以檢討。

1. The Watson and Crick model of a double-helical structure for DNA was based on evidence from (A) Atomic force microscopy. (B) Cryo-EM (electron microscopy). (C) NMR (nuclear magnetic resonance) spectroscopy. (D) X-ray crystallography.

2. Nucleotides in an RNA molecule are linked together through (A) Amide bonds. (B) Glycosidic bonds. (C) Hydrogen bonds. (D) Phosphodiester bonds.

4. Which of the following statements about the polymerase chain reaction (PCR) is false? (A) PCR is a technique to make many copies of a specific DNA fragment in vitro. (B) PCR requires one primer designed specifically for the DNA region of interest. (C) PCR relies on a thermostable DNA polymerase, Taq polymerase. (D) PCR is routinely used in DNA cloning, medical diagnostics, and forensic analysis of DNA.

5. The transcriptome is the set of all RNA molecules in one cell or a population of cells. Which of the following methods can be used for transcriptome analysis? (A) DNA microarray. (B) RNA sequencing (RNA-Seq). (C) Serial analysis of gene expression (SAGE). (D) All of the above.

6. When bound to DNA, repressor proteins usually prevent attachment of RNA polymerase to the _____.(A) operon. (B) operator. (C) promoter. (D) regulator.

7. _____ molecules deliver amino acids to ribosomes during translation. (A) Messenger RNA (mRNA). (B) Transfer RNA (tRNA). (C) Ribosomal RNA (rRNA). (D) Heterogeneous nuclear RNA (hnRNA).

8. Which of the following effects may be mediated by transposable elements? (A) Insertion into a gene, causing a mutation. (B) Activation of nearby genes. (C) Formation of genetic deletions. (D) All of the above.

9. ORF refers to (A) the location of the start site of transcription. (B) the region of a gene that codes for a protein or a functional RNA product. (C) the region of a gene that serves as the promoter. (D) the region between the ribosome binding site and translation initiation codon.