1. You are trying to separate proteins A, B, C, D, and E by gel filtration chromatography: Which of the
proteins will be eluted in the second order from the column?
(A) protein A (42 kDa)
(B) protein B (99 kDa)
(C) protein C (64 kDa)
(D) protein D (121 kDa)
(E) protein E (82 kDa)