11. Which answer represents the correct order of clectron flow between 1 to 4 factors?

 1. Cytochrome c 2. Co-enzyme Q 3. Oxygen 4.NADH

 
(A) 4,3,2, 1
(B) 4, 1,2,3
(C) 4, 2,1, 3
(D) 2, 1,4, 3 
(E) 2, 4, 1, 3

7. Which of the following is the major advantage of a multi-enzyme complex? (A) It's large size enables it to span an entire membrane. (B) The product of one enzyme is passed directly to the next enzyme without the possibility of difiusion. (C) Multi-enzyme complexes are much less likely to be inhibited. (D) All of the above. (E) None of the above.

8. Glycogen phosphorylase displays allosteric activation and inhibition by multiple modes. Which of the following is a correct relation? (A) Phosphate: positive heterotropic effector. (B) AMP: negative heterotropic effector. (C) ATP: positive heterotropic effector. (D) Glucose-6-phosphate: negative heterotropic effector. (E) Phosphorylation: covalent inhibitor.

9. All are characteristics of molecular motors or motor proteins except: (A) They must be able to associate and dissociate reversibly with a polymeric protein array, a surface or substructure in the cell. (B) They use chemical energy (e.g., ATP) to orchestrate movement. (C) They transfer ATP energy into mechanical energy. (D) ATP bydrolysis is presumed to drive and control protein conformational changes that result in sliding or walking movement of one molecule relative to another. (E) All are true.

10. Which of the following statement regarding Cori cycle and pentose phosphate pathway is false? (A) Cori cycle involves in conversion of lactate produced in the muscle by regeneration of glucose in the liver. (B) Cori cycle involves in conversion of lactate produced in the liver by regeneration of glucose in the muscle. (C) The pentose phosphate pathway provides precursors for the synthesis of nucleotides. (D) The metabolic function of the pentose phosphate pathway is to generate NADPH and pentoses for the biosynthesis of fatty acids and nucleic acids (E) All are correct.

12. Which of the following statements about pyruvate metabolism is correct?  1. △G° of hydrolysing phosphoenopyruvate to pyruvate is negative. 2.Under anaerobic condition, the purpose of reducing pyruvate to lactate is to regene  3. Conversion of glyceral dehyde-3-hosphate to pyruvate involves enolase and pyruvate kinase.  4. The pyruvate dehydrogenase complex uses all of the following as cofactors: NAD+, COA, FAD, lipoic acid, and thiamine pyrophosphate (TPP).  5. Pyruvate carboxylase requires biotin for activity.  (A) 1, 2, and 3 are correct. (B) 1, 3, and 5 are correct. (C) 2, 3, and 4 are correct.(D) 2, 4, and 5 are correct. (E) All are correct.

13. Which of the following statements regarding citric acid cycle or glyoxylate cycle is false?  1. One round of citric acid cycle can generate 10 ATP. 2. The catabolism of proteins, carbohydrates, and fatty acids all feed into the citric acid cycle at one or more points.  3. The 2nd step of glyoxylate cycle uses malate synthase to convert 2 glyoxylate to I malate molecule.  4. Glyoxylate cycle takes place in glyox ysomes during plant cells are performing photosynthesis. (A) 1 and 2 are false. (B) 3 and 4 are false. (C) 2 and 3 are false. (D) 2 and 4 are false. (E) None of above is false.

14. NADH,_______ and__________ are products of glycolysis, and the NADH must be recycled to ________before it becomes limiting in glycolysis. (A) ATP; pyruvate; NAD+ (B) NAD+; ATP; pyruvate (C) ATP; NAD+; ATP (D) ATP; pyruvate; lactate (E) None are true.

15. The endoplasmic reticulum bound enzyme that hydrolyzes glucose-6-phosphate to glucose in liver is: (A) glucokinase. (B) glucose oxidase. (C) hexokinase. (D) phosphoglucomutase. (E) glucose-6-phosphatase.

16. If both NADPH and ribose-5-P are needed in the cells which of the following best represents the net reaction of the pentose phosphate pathway? (A) 4 Fruc-6-P +2 glyceraldehyde-3-P →6 ribose-5-P (B) 3 Gluc-6-P +6 NADP+ →6 NADPH + 3 CO2 + 2 Fruc-6-P + 1 glyceraldehyde-3-P + 6 H+ (C) Gluc-6-P + 2 NADP+ +2 H2O → 2 NADPH + CO2 + ribose-5-P +2 H+ (D) Gluc-6-P + 12 NADP+ +6 H2O →12 NADPH +. 6 CO2 + 12 H+ + Pi (E) None of the above.

17. Shuttle vectors have the property that they: (A) contain promoters for the expression of the gene. (B) have origins of replication for two different cell types, usually bacteria and yeast. (C) are capable of incorporating very large DNA fragments. (D) contain more than one antibiotic resistant gene. (E) None of the above.