12. Which of the following statements about pyruvate metabolism is correct? 1. △G° of hydrolysing phosphoenopyruvate to pyruvate is negative. 2.Under anaerobic condition, the purpose of reducing pyruvate to lactate is to regene 3. Conversion of glyceral dehyde-3-hosphate to pyruvate involves enolase and pyruvate kinase. 4. The pyruvate dehydrogenase complex uses all of the following as cofactors: NAD+, COA, FAD, lipoic acid, and thiamine pyrophosphate (TPP). 5. Pyruvate carboxylase requires biotin for activity. (A) 1, 2, and 3 are correct. (B) 1, 3, and 5 are correct. (C) 2, 3, and 4 are correct.(D) 2, 4, and 5 are correct. (E) All are correct.