13. Diacylglycerol activates which of the following enzymes?
(A) Protein kinase A
(B) Protein kinase C
(C) Phosphorylase b kinase
(D) Tyrosine kinase

1. Phosphorylation is an important covalent modification of cellular proteins, which can regulate the activity of many proteins. Protein phosphorylation is mediated by protein kinases in the present of ATP/. If you want to -label a substrate protein by its upstream kinase, what kind of ATP should be used in the reaction? (A) (B) (C) (D)

2. Dialysis bag is usually used for concentrating proteins from diluted biological samples. The dialysis bag has different pore sizes, and choice of pore size of dialysis bag is dependent on the molecular weight of the protein to be concentrated. If you want to concentrate a protein having 50 amino acid residues, what pore size of dialysis bag can not be used? (A) 1000 (B) 2000 (C) 4000 (D) 8000

3. A synthetic peptide has the following amino acid sequence: GYTVLKEEDFLSWRIMCQQNIGAR. If this peptide is digested completely by trypsin, how many fragments can be generated? (A) 1 (B) 2 (C) 3 (D) 4

4. What kind of solution is usually used to disrupt the cell membrane of mammalian cells? (A) Phosphate buffer saline (B) 0.1% EtBr (C) 0.1% Triton X-100 (D) 1% HCl

5. What kind of reagent is the most sensitive one to detect proteins resolved in SDS-gel? (A) Silver Nitrate (B) EtBr (C) Coomassie blue (D) Amido Black

6. "Catalytic antibody" is a kind of antibody with enzyme activity that can trigger the following reaction: A→B→C, where A, B and C represent reactant, intermediate and product, respectively. What is the antigen that is used to generate this "catalytic antibody"? (A) A (B) B (C) C (D) A+B+C

7. The structure of myoglobulin contains a protoporphyrin group, which is used to bind oxygen. Several important amino acid side chains are required to hold the protoporphyrin group and oxygen inside the myoglobulin. Please point out what is the amino acid side chain indicated by an arrow (see below) in this core structure? (A) Tyr (B) Phe (C) His (D) Thr

8. Antibodies are important research tools in biomedical research. If you want to determine the molecular weight of a specific antigen, what kind of experiment is the most suitable one to get this information? (A) Northern Blot (B) Immunofluorescence (C) EMSA (D) Western Blot

9. Shown here is a sigmoid (cooperative) binding curve when hemoglobin binds oxygen in the presence of different pO2. Please calculate how may percentage of oxygen can be released from hemoglobin when the pO2 is reduced from 8 kPa to 2 kPa. (A) 40% (B) 50% (C) 60% (D) 70%

10. In β-oxidation, long-chain fatty acids are activated to acyl-CoAs and the acyl group is further transferred to carnitine because: (A) Fatty acids can not be oxidized by acyl-CoA dehydrogenase unless they are in the acyl-carnitine form. (B) Acyl-CoAs easily cross the mitochondrial membrane, but the fatty acids themselves will not. (C) Carnitine is required to oxidize to NADH. (D) Acyl-carnitines readily cross the mitochondrial inner membrane, but acyl-CoAs do not.

115年 - [無官方正解]115 國立臺灣大學_ 碩士班招生考試試題：生物化學(一般生物化學)#137622

115年 - 115 國立臺灣大學_ 碩士班招生考試試題：生物化學(B)#137619

114年 - [無官方正解]114 國立清華大學_碩士班考試入學試題_生命科學暨醫學院甲組(生物與醫學科學組)﹕生物化學#132802

114年 - [無官方正解]114學年度學士後獸醫學系招生試題-生物化學#131167

114年 - [無官方正解]114 台灣綜合大學系統_學士班轉學生聯合招生考試：生物化學#129347

114年 - [無官方正解]114 國立臺灣大學_轉學生招生考試試題：生物化學概論#128196

114年 - 114 台灣聯合大學系統_碩士班招生考試試題_化學類：生物化學#126447

114年 - 114 國立成功大學_碩士班招生考試試題：生物化學#126226

114年 - 114 國立臺灣大學_ 碩士班招生考試試題：生物化學(C)#125975

114年 - 114 國立臺灣大學_ 碩士班招生考試試題：生物化學(A)#125974