試題詳解

15. The production of recombinant DNA (DNA cloning) involves the following 4 steps. Please put them into correct experimental order.

 I. Insertion of foreign DNA fragment into a cloning vector.

II. Preparation of fragments of vector and foreign DNA with compatible ends.

III. Introduction of recombinant DNA into a recipient cell for replication.

IV. Selection of cells containing the correct recombinant DNA.
(A) I→II→III→IV
(B) II→I→III→IV
(C) II→III→I→IV
(D) II→III→I→IV
(E) II→I→III→IV