16. Which of the following is true regarding the Briggs and Haldane steady state assumption? (A) It is defined by the equation E + S←→ ES ←→ E + P, (B) It states the rate of enzyme-substrate complex formation differs from the rate of enzyme-substrate disappearance, (C) The concentration of the enzyme-substrate complex reaches a constant value even in a dynamic system, (D) The enzyme-substrate complex will always dissociate to form E + P, (E) The total amount of enzyme is variable, depending on the amount of substrate available.