試題詳解

22. What the followings are True when one design a set of primer for cloning:
(A) Introducing extra sequences that would give us a restriction site in primer can be added at either 5' or 3' end of primer
(B) Annealing temperature should only consider the sequence that could based paired with the target gene
(C) Only if a N-terminus protein tag has been introduced into vector backbone, one should consider whether introduced restriction sites in the primer result in frame shift in the final protein products
(D) Length of primer can be as short as 5 nucleotides
(E) As long as melting temperature reaches to the target temperature, nucleotide composition is not affect the primer efficiency