26. Mr. Bean synthesizes a pentapeptide "DIRTY". Choose the correct answer from the following descriptions (To calculate pl, use the pK2 of the N-terminal amino acid residue as the pKa of the N-terminal amino group of the peptide and pK, of the C-terminal amino acid residue as the pKa of the C-terminal carboxylate group of the peptide):
1. The pl value of the peptide is 6.625.
2. The pl value of the peptide is 2.925.
3. The pl value of the peptide is 6.38.
4. When 0.1 mg of the peptide is dissolved in 100 mM Tris (pH 9), it could bind to anion exchange column.
5. When 0.1 mg of the peptide is dissolved in 100 mM Tris (pH 9), it could bind to cation exchange column.

(A) 1 and 4 are correct.
(B) 2 and 4 are correct.
(C) 1 and 5 are correct.
(D) 3 and 4 are correct.

(E) 3 and 5 are correct.

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