試題詳解

39. Which of the following experiments may help Lisa gain insights into her project?
(A) Compare the fly mutation to existing vertebrate mutations in the homologous genes and see whether the mutation affects conserved residues of the protein
(B) Sequence the entire genome of the mutant fly to find any mutations that may co-exist with the small GTPase mutation
(C) The initial mapping is wrong and she should re-map the mutation all over again
(D) Perform RNAi against this small GTPase in the mutant flies, rather than in wild type flies, to exclude the possibility that it is a neomorphic mutation
(E) Purify the mutant protein by expressing it in the bacterial system, and perform in vitro GTPase assay to understand how the protein activity is affected