試題詳解

39. Which of the following experiments may NOT help Jared gain support for his hypothesis?
(A) Compare the protein contents in unfertilized oocytes and fertilized oocytes; proteins existing in the latter but not in the former could be candidates for cell division-promoting factors.
(B) Analyze the sperm proteins. Since the oocyte begins to divide after fertilization, the key element is in the cytoplasm of the sperm.
(C) Separate the nucleus, cell membrane as well as the cytoplasm of fertilized oocytes, and add each of these individual components to the non-dividing cells. The prediction is that the fertilized oocyte cytoplasm should induce cell division when added to other non-dividing cells.
(D) Do a pulse-labeling of newly synthesized proteins in the fertilized oocytes with radioisotopes, and compare protein radioactivities in the dividing v.s. non-dividing cells. This may help identify proteins that are associated with cell division cycles.
(E) Do a genome-wide RNAi screen to identify the gene encoding the cytoplasmic factor that Jared is looking for.