試題詳解

5.A researcher is using degenerate primers to amplify a gene from a newly discovered organism. They designed the primers based on a short peptide sequence from a protein that they think is encoded by the gene. What is the most likely reason that a pool of different primers is needed for PCR amplification in this scenario?
(A) The use of degenerate primers ensures that the PCR reaction has multiple binding sites on the DNA template, thus amplifying the sequence more efficiently.
(B) The use of degenerate primers ensures the amplification of multiple related. genes.
(C) Many amino acids are encoded by multiple codons, so there are several. possible DNA sequences that could code for the same peptide sequence.
(D) Degenerate primers help overcome the mispriming that can occur during PCR.
(E) Degenerate primers increase the specificity of the PCR reaction.