試題詳解

6. When Samuel is doing protein purification for a mixture of three proteins (protein A with pI 4, protein B with pI 9, protein C with pI 7), which statement in the following is       correct      ?
(A) When the mixture is adjusted to pH 6, only protein A can bind in an anion exchange column.
(B) When the mixture is adjusted to pH 8, protein B can not bind in a cation exchange column.
(C) When the mixture is adjusted to pH 10, the elution sequence in a gel filtration column is A-C-B.
(D) When the mixture is adjusted to pH 8, only protein B can bind in an anion exchange column.
(E) When the mixture is adjusted to pH 10, all three proteins can not bind in an anion exchange column.