試題詳解

8. A graduate student accidentally cloned a cDNA from an economically important animal whose genomic sequence has not been resolved. The sequence of this cDNA can be summarized as below:

5'-gegatagtataatatcggccaggATGccg..ggccgggtattctcatttTAAggcaggttctta-3 The start and stop codons are shown in bold capital. If he/she likes to amplify the coding region ofthis gene, which one of the following PCR primer sets will work for him/her?
(A). 5'-cggccaggATGccg-3'/5'-ttctcatttTAAggcaggttctta-3
(B). 5'-gccggtcCTAcggc -3'/5'-ttctcatttTAAggcaggttctta-3
(C). 5'-cggccaggATGccg-3'/5'-taagaacctgccTTAaaatgagaa -3
(D). 5'-gccggtcCTAcggc -3'/ 5'-aaaATTccgtccaagaat -3