試題詳解

9. Which of the following limits the use of PCR to detect and isolate genes?
(A) The sequence at the beginning and end of the DNA to be amplified must be known.
(B) It also produces large numbers of copies of sequences beyond the 5' or 3' end of the desired sequence.
(C) It cannot be used to amplify cDNAs or mRNAs.
(D) It will amplify only sequences present in multiple copies in the DNA sample.
(E) Quantitation can be applied by adding modified fluorescent nucleotides.