9. Which of the following limits the use of PCR to detect and isolate genes?
(A) The sequence at the beginning and end of the DNA to be amplified must be known.
(B) It also produces large numbers of copies of sequences beyond the 5' or 3' end of the desired sequence.
(C) It cannot be used to amplify cDNAs or mRNAs.
(D) It will amplify only sequences present in multiple copies in the DNA sample.
(E) Quantitation can be applied by adding modified fluorescent nucleotides.

5, Which of the following transposons does not contain inverted repeats or LTRs at its ends? (A) Tn3 (B) Ds (C) copia (D) Ty (E) Alu elements

6. Sarah has performed a chromatin immunoprecipitation experiment using a specific antibody that recognizes a specific protein that can bind to TATA box in E. coli. This protein is likely (A) TFIID (B) RNA polymerase-II (C) Sigma factor (D) TBP (E) Helicase

7. Which of the following mechanism is most used to repair a single stranded DNA break? (A) Mismatch repair (B) Homologous recombination (C) Nucleotide excision repair (D) Base excision repair (E) Non-homologous end joining

8. Which modified nucleotide base is part of the 5'cap on eukaryotic mRNAs?(A) cytosine(B) guanine(C) adenine(D) uracil(E) thymidine

10. Unlike DNA, which typically forms a helical structure, different molecules of RNA can fold into a variety of three-dimensional shapes. This is largely because_________. (A) RNA contains uracil and uses ribose as the sugar. (B) RNA bases cannot form hydrogen bonds with each other. (C) RNA nucleotides use a different chemical linkage between nucleotides compared to DNA. (D) RNA is single-stranded.

11. Put the following steps of prokaryotic translation initiation in the correct order.(1) Binding of IF1, IF2, and GTP to the 30S subunit.(2) Binding of IF3 to the 30S subunit.(3) Binding of the 50S subunit and loss of IF1 and IF3.(4) Dissociation of the 70S ribosome.(5) Formation of the 70S initiation complex by dissociation of IF2 and GTPhydrolysis.(6) Formation of the 30S initiation complex.(A) 1,2,3,4,5,6(B) 2,1,6,3,5,4(C) 2,4,1,6,3,5(D) 4,2,1,6,3,5(Ε) 5,6,3,2,1,4

12. The Kozak sequence on an mRNA molecule is recognized by the ribosome as the _______in eukaryotes. (A) ribosomal binding site (B) interacting site with 5' cap (C) translational start site (D) internal ribosome entry site (E) ribosome exit ( E ) site

13. Which of the following initiation factor is involved in aiding in ribosome scanning to locate the initiation codon? (A) eIF1 (B) eIF2 (C) eIF3 (D) eIF4F (E) eIF6

14. Picornavirus mRNAs are not capped, yet they can still out compete host mRNAs for binding to the ribosome by (A) degrading host mRNA. (B) inactivating host Cap binding protein, eIF4F.. (C) having a stronger affinity for the ribosome. (D) inactivation of host RNases. (E) inactivation of host eIF2B.

15. Place the steps of translation elongation in the correct order.(1) Peptidyl transferase forms a peptide bond between the peptide in the P site and the newly arrived aminoacyl-tRNA in the A site.(2) EF-G, with GTP, translocates peptidyl-tRNA to the P site.(3) EF-Tu, with GTP, binds an aminoacyl-tRNA to the ribosomal A site.(A) 1, 2, 3(B) 2, 3, 1(C) 1, 3, 2(D) 3, 2, 1(E) 3, 1, 2