申論題

7. (15%) Enzymes serve as catalysts in many chemical reactions in living systems. The simplest such reactions transform a single substrate into a product with the help of an enzyme, which may be represented schematically as

In this mechanism, the enzyme E is a catalyst, which only facilitates the reaction, so that its total concentration, free plus combined, [E] + [ES] = [E]o is a constant.

(c) (4%) Show that the Michaelis-Menten equation can be written in the form
(1/V₀)=(K_m/V_max)(1/[S]₀) + (1/V_max)
(This formula is know as the Lineweaver-Burk equation and shows that there is a linear relationship between (1/V₀) and (1/[S]₀).)