申論題

7. (15%) Enzymes serve as catalysts in many chemical reactions in living systems. The simplest such reactions transform a single substrate into a product with the help of an enzyme, which may be represented schematically as

In this mechanism, the enzyme E is a catalyst, which only facilitates the reaction, so that its total concentration, free plus combined, [E] + [ES] = [E]o is a constant.

(d) (4%) Sketch the graph ofthe lineweaver-Nurk equation. Use a coordinate system in which (1/[S]₀) is on the horizontal axis and (1/V₀) is on the vertical axis. Show the resulting graph is a line that intersects the horizontal axis at (-1/K_m) and the vertical axis at (1/V_max)