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114年 - B5 Lesson 01：Life Lessons in Rudyard Kipling's "If—"_文意選填+篇章結構+混合題#128850
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(二)請回答下列問題1. The tertiary structure of protein may be studied by four techniques, x-ray crystallography, NMR, electron microscopy and mass spectroscopy. Please describe the principles behind these techniques. Please discuss their advantages and drawbacks. Similarities exist between NMR and mass spectroscopy when they are used for structure analysis, please explain why?
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2. What are the advantages to the plant cell of having different organelles to carry out different reaction sequences that share intermediates? Please employ the following terms in your discussion, ATP/ADP ratio, NADH/NAD+.
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3. Please explain how Scatchard analysis is performed to study receptor-ligand interaction.
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4. If cholesterol, phosphatidylglycerol and beeswax were dissolved in chloroform, then subjected to thin-layer chromatography on silica gel using a mixture of chloroform/methanol/water as the developing solvent, which would move slowest? Why?
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5. Please describe the reaction catalyzed by acetyl-CoA carboxylase in lipid biosynthesis and explain the biochemical role of its covalently bound prosthetic group.
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