33. Using the figure on right, what is the first bond broken in the base excision repair process?

4. Continuing the question above, please draw the structure of dideoxy form of the structure above.  A peptide sequence, "YPYDVPDYA," which is derved fom a viral protein and plays a role in bing the virus to cell rectorerrrr to initiate infection, has recently been identified. Please answer the following questions.

6. What is the complementary sequence of the sequence above?

27. Describe the allosteric regulation of hemoglobin. How does this regulation facilitate oxygen delivery to tissues?

28. Name two enzymes and explain how their names reflect their function or substrate.

29. Draw a schematic diagram illustrating the signal transduction pathway of a G-protein coupled receptor (GPCR).

31. You want to amplify the DNA between the two stretches of sequences shown in Figure below. Of the listed primers choose the pair that will allow you to amplify the DNA by PCR.

32. In Figure below, the bacterial activator protein CAP and the Lac repressor have been placed in the four possible combinations on their binding sites in the promoter for the Lac operon. Each combination of gene regulatory proteins corresponds to a particular mixture of glucose and lactose. For each of the four combinations, indicate on the left-hand side of the figure which sugars (glucose or lactose) must be present (or absent) and, on the right-hand side, whether the operon is expected to be turned ON or OFF.

34. The E. coli chromosome contains 4,639,221 base pairs. How long would it take to replicate the E. coli chromosome at 37 °C if two replication forks proceed from the origin? Assume replication occurs at a rate of 1,000 base pairs per second. Under some conditions E. coli cells can divide every 20 min. How might this be possible? 

35. What is the biosynthesis of heme?

34. What is the process of glycogen biosynthesis from glucoses?

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