8. There are different platforms of next-generation sequencing: pyrosequencing, sequencing by ligation, sequencing by synthesis, SMRT sequencing and semiconductor sequencing. Pick one and describe how it works. (4%)

2. In Figure below, the bacterial activator protein CAP and the Lac repressor have been placed in the four possible combinations on their binding sites in the promoter for the Lac operon. Each combination of gene regulatory proteins corresponds to a particular mixture of glucose and lactose. For each of the four combinations, indicate on the left-hand side of the figure which sugars must be present and, on the right-hand side (a-d), whether the operon is expected to be turned ON or OFF. (4%)

8. There are different platforms of next-generation sequencing: pyrosequencing, sequencing by ligation, sequencing by synthesis, SMRT sequencing and semiconductor sequencing. Pick one and describe how it works. (4%)

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