35. In addition to the Transposase Gene, what is the most important component of DNA transposons?
(A) Inverted Repeats
(B) Long Terminal Repeats
(C) Direct Repeats
(D) Internal Resolution Site
(E) Resolvase Gene

31. Which of the following correctly differentiates rolling circle replication from binary replication in DNA replication? (A) Rolling circle replication involves bidirectional replication, while binary replication occurs unidirectionally. (B) Rolling circle replication is primarily used in prokaryotic chromosomal replication, whereas binary replication is exclusive to plasmid DNA. (C) Rolling circle replication generates single-stranded intermediates, while binary replication replicates both DNA strands simultaneously. (D) Rolling circle replication is initiated at multiple origins of replication, whereas binary replication starts at a single origin. (E) Rolling circle replication requires Okazaki fragments, while binary replication does not.

32.Which enzyme is commonly added when using a single restriction enzymein cloning, and what is its purpose?(A) T4 DNA polymerase, to fill in 5' overhangs and generate blunt ends toincrease ligation efficiency.(B) Reverse transcriptase, to synthesize cDNA from RNA prior to cloning.(C) Exonuclease, to degrade the unwanted DNA after restriction enzymedigestion.(D) Alkaline phosphatase, to prevent the vector from self-ligating by removing5' phosphate groups.(E) Topoisomerase, to relieve supercoiling in the DNA fragment during cloning.

33. Which of the following statements about PCR is incorrect? (A) Quantitative PCR can directly quantify the relative concentration of mRNA. (B) The use of 95°C during the reaction process is to cause DNA denaturation. (C) The temperature setting for the annealing step will affect the specificity of the product. (D) The use of 72°C during the reaction process is for polymerization, and the time must be adjusted based on the product size. (E) The discovery of Taq DNA polymerase, which can withstand high temperatures, is the key to PCR technology.

34. Integration of Lamda phage into host genome is mediated by a process called:(A) Cut-and-paste transposition(B) Replicative transposition(C) Homologous recombination(D) Non-homologous end-joining(E) Site-specific mutagenesis

1. The opposite meaning of “increase” is:(A) Expand(B) Rise(C) Decrease(D) Grow

2. In design, "contrast” means:(A) Similarity(B) Difference(C) Repetition(D) Harmony

3. "Freelancer" refers to:(A) A person working full-time in one company(B) A person studying(C) A person who doesn't work(D)A person who is self-employed and not necessarily committed to a particular employer long-term

4. "Resolution” in images means:(A) The clarity of a picture(B) The size of a file(C) The type of a color(D) The style of a design

5. Which phrase means “right away"?(A) At once(B) In advance(C) By chance(D) From scratch

6. What does the idiom "break the ice" mean?(A) To literally break frozen water(B) To make people who have not met before feel more relaxed with each other(C) To make someone angry(D) To cancel a plan