81. Which of the following is the major site of expression for the monosaccharide transporter GLUT1?
(A) erythrocytes, CNS, blood brain barrier
(B) liver, pancreaticβ-cells, small intestine
(C) neurons, testis, placenta
(D) skeletal muscle, adipose tissue
(E) small intestine, kidney, skeletal muscle, adipose tissue

77. The technique known as yeast two hybrid analysis for detecting interacting gene products depend on: (A) activation of DNA polymerase by the nearby binding of hybridizing protein complexes. (B) direct binding of a Gal4p activation domain to a DNA sequence in the promoter region. (C) having a promoter that responds directly to one of the two proteins whose interactions is being measured. (D) hybridization of DNA segments corresponding to the two genes being examined. (E) stimulation of transcription by interaction of two Gal4p domains via fused protein sequences.

78. In DNA sequencing by the Sanger (dideoxy) method: (A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands. (B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis. (C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence. (D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands. (E) the template DNA strand is radioactive.

79. Which of the following compounds would be most closely associated with the intermediate common to glycogenesis and glycogenolysis? (A) 6-phosphogluconate (B) Glucose-1-phosphate (C) Glucose-6-phosphate (D) Fructosc-6-phosphate (E) Dihydroxyacetone phosphate

80. Which of the following enzymes and pathways are correctly matched? (A) Phospholipase D and prostaglandin synthesis (B) HMG-CoA reductase and ketone body synthesis (C) Cyclooxygenase and thromboxane synthesis (D) Ethanolamine kinase and phosphatidylcholine synthesis (E) Phospholipase A2 and ceramide synthesis.

82. Which of the following is/are the precursor(s) for the amino acid arginine? (A) glutamate, ammonia (B) glutamate (C) phenylalanine (D) methionine, serine (E) glutamine, or glutamate, aspartate

83. Fluroacetate is an inhibitor of aconitase in TCA cycle. Which of the following types of inhibition does fluroacetate belong to? (A) Irreversible inhibition (B) Competitive reversible inhibition (C) Mixed reversible inhibition (D) Uncompetitive reversible inhibition (E) None of the above

84. The complete oxidation of I mole of glucose generates about 30-32 moles of ATP synthesized from ADP. What is the reason for 2 moles ATP difference? (A) The reducing equivalent FADH2 is shuttled from cytosol into mitochondria via the dihydroxyacetone phosphate/glycerol-3-phosphate shuttle (B) The reducing equivalent NADH is shuttled from cytosol into mitochondria via the dihydroxyacetone phosphate/glycerol-3-phosphate shuttle (C) The reducing equivalent FADH2 is shuttled from cytosol into mitochondria via the malate/aspartate shuttle (D) The reducing equivalent NADH is shuttled from cytosol into mitochondria via the malate/aspartate shuttle (E) The reducing equivalent ATP is shuttled from cytosol into mitochondria via the malate/aspartate shuttle

85. If glucose labeled with 14C in C-1 were fed to yeast carrying out the ethanol fermentation, where would the 14C label be in the products? (A) In C-1 of ethanol and CO2 (B) In C-1 ofethanol only (C) In C-2 (methyl group) of ethanol only (D) In C-2 of ethanol and CO2 (E) In COz only

86. Which of the following statements concerning the B oxidation of fatty acids is true? (A) About 1,200 ATP molecules are ultimately produced per 20-carbon fatty acid oxidized. (B) One FADH2 and two NADH are produced for each acetyl-CoA. (C) The free fatty acid must be carboxylated in the B position by a biotin-dependent reaction before the process of B oxidation commences. (D) The free fatty acid must be converted to a thioester before the process of B oxidation commences. (E) Two NADH are produced for each acetyl-CoA.

87. Which one of the following statements about the reverse transcriptases of retroviruses and the RNA replicases of other single-stranded RNA viruses, such as R17 and influenza virus, is correct? (A) Both enzymes can synthesize either RNA or DNA from an RNA template strand. (B) Both enzymes can utilize DNA in addition to RNA as a template strand. (C) Both enzymes carry the specificity for the RNA of their own virus. (D) Both enzymes have error rates similar to those of cellular RNA polymerases. (E) Both enzymes require host-encoded subunits for their replication function.