105年 - 105 臺灣綜合大學_學士班轉學生聯合招生考試試題：分子生物學#119790

科目：研究所、轉學考(插大)◆分子生物學 | 年份：105年 | 選擇題數：30 | 申論題數：8

試卷資訊

所屬科目：研究所、轉學考(插大)◆分子生物學

選擇題 (30)

1.The strand on which DNA replication is continuous is called the: (A) Leading strand (B) Lagging strand (C) Major strand (D) Minor strand

2.When the gene pair in which both the maternal and paternal genes are identical (For example, RR or rr), it is called: (A) dominant (B) recessive (C) homozygous (D) heterozygous

3.On a polypeptide, which amino acid cannot be phosphorylated? (A) serine (B) valine (C) threonine (D) tyrosine.

4.Which of the followings is a stop condon? (A) UGG (B) UGA (C) UUA (D) AUG

5.Which of the followings does not belong to core histones? (A) HI histone (B) H2A histone (C) H3 histone (D) H4 histone

6.In E. coli, DNA polymerase I does not have which of the following activity? (A) 5' to 3' polymerase activity (B) 3' to 5' polymerase activity (C) 5' to 3' exonuclease activity (D) 3' to 5' exonuclease activity.

7. Which of the following is a true statement regarding DNA polymerase? (A) It is a processive enzyme (B) It contains proofreading activity (C) It requires a short primer or oligonucleotide to start synthesizing new DNA strands (D) All of the above

8. Which of the following does not play a role in DNA replication? (A) DNA polymerase (B) Helicase (C) Single-stranded binding protein (D) Guanylyltransferase

9.If you want to detect the RNA of a specific gene expressed in different tissues (heart, lung, brain, what technique would you use? (A) Southern blotting (B) Northern blotting (C) Western blotting (D) South-Western blotting

10. In E.coli, which of the following protein is responsible for detecting mismatched DNA? (A)MutL (B) MutH (C) MutJ (D) MutS

11. Which subunit of E.coli DNA polymerase III increases its processivity? (A)a (B) B (C) 8 (D)y

12. Which of the following molecules is involved in DNA nucleotide excision repair? (A) RecBCD (B) MutH (C) UvrC (D) Spoll

13. Which of the following components is not used during PCR? (A) DNA Polymerase (B) Oligonucleotide Primers (C) DNA Ligase (D) Template DNA

14. The aromatic rings of the bases found in DNA and RNA absorb ultraviolet light with an absorption maximum at: (A) 240 nm (B) 260 nm (C) 280 nm (D) 300 nm

15. Which of the following molecules is not involved in homologous recombination? (A) RecBCD(B) RecA (C) RuvA (D) UvrA

16. The pre-miRNA was processed into miRNA by? (A) Dicer (B) Parsha (C) Argonaute (D) Drosha

17. The CTD domain of RNA polymerase can be phosphorylated by which kinase during the initiation step of transcription? (A) TFIB (B) THID(C) pTEFb (D) TFIH

18. Following the previous question, which kinase is involved in the clongation step of transcription? (A)TFIIB (B) TFID (C) pTEFb (D) TFIH

19. Which enzyme is NOT involved in RNA editing? (A) exonuclease (B) endonuclease (C) deaminase (D) uridylyltransferase

20. Which enzyme does play a role in polyadenylation of mRNA? (A) RNA ligase (B) RNA triphosphatase (C) GuanylyItransferase (D) Cleavage stimulation factor CstF

21. What kind of amino acids are involved in the interaction with zinc iron of zinc finger proteins? (A) Serine and Threonine(B) Cysteine and Histidine (C) Asparagine and Glutamine (D) Aspartic acid and Glutamic acid

22. Which protein contains the helicase activity in translation? (A)eIF4G (B) eIF4A (C)eIF4B (D) elF4E

23. Which protein complex for transcription does NOT contain TBP? (A)SL1 (B) TFIID (C) TFIE (D) TFIIB

24. Which transcription factor was NOT proved to be able of reprogramming somatic cells into embryonic stem cells? (A) c-Myc (B) TBP (C) Oct4 (D) Sox2

25. Which sequence is the ribosome binding site in the prokaryotic transcript? (A) 5'-UTR (B) Kozak sequence (C) Shine-Dalgarno sequence (D)3'-UTR

26. In the infection of E.coli by λ phage, which protein could orchestrate the choice between lytic and lysogenic development? (A) cro (B) cl (C) cII (D) cIll

27. Which assay was used for identifying the interacting protein? (A) EMSA (electrophoretic mobility-shift assay) (B) Western blotting (C) CHIP( chromatin immunoprecipitation assay) (D) Yeast two-hybrid assay

28. Which domain is NOT classified as the DNA-binding domain of a transcriptional activator? (A) Acidic domain (B) Homeodomain (C) Leucine zipper (D) Helix-loop-helix

29. Which region of sigma factor of prokaryotic RNA polymerase can recognize the -10 region of promoter? (A) region 1.1 (B) region 2.3 (C) region 2.4 (D) region 4.2

30. Which factor does NOT belong to the family of GTPase in translation? (A) IF2 (B) EF-G (C) EF-Ts (D) RRF (ribosome recycling factor)

申論題 (8)

a. DNA glycosylase

b. Spoll

c. RAG1 and RAG2

d. Helicase

e. Telomerase

32. Please describe the detailed process of bomologous recombination in E. coli (10 points)

33. Please describe the Trp attenuation of Trp operon in prokaryote. (8 points)

34. Please compare the three eukaryotic RNA polymerases, including the gene products, the protein factors and the promoter regions. (12 points)