110年 - 110 國立台灣海洋大學_碩士班招生考試_食品科學系碩士班生物科技組：分子生物學#103776

科目：研究所、轉學考(插大)◆分子生物學 | 年份：110年 | 選擇題數：17 | 申論題數：13

試卷資訊

所屬科目：研究所、轉學考(插大)◆分子生物學

選擇題 (17)

2. Single choice questions.

(1) Which of the followings is most likely to bind to phosphorylated tyrosine? (A) TATA box proteins (B) Nucleobases (C) Src homology 2 domain (D) Random coils (E) Helix-turn-helix motif

(2) Which of the followings is not true regarding RNA? (A) Can form tertiary structure. (B) Single-stranded. (C) Can catalyze chemical reactions (D) Has the sugar ribose (E) Does not have Watson-Crick base pairs

(3) Which of the followings regarding A-to-I RNA editing is incorrect? (A) Co-transcriptional (B) Chemical modification of RNA (C) Catalyzed by Adenosine DeAminase acting on RNA enzymes (D) Relatively frequent in human than in other organisms (E) Reversible

(4) Which of the followings regarding GC content is not true? (A) is the percentage of guanine and cytosine bases in a DNA or RNA molecule (B) not identical in all living organisms (C) A reason for codon usage bias (D) DNA with low GC-content is stable than DNA with high GC-content (E) None of the above

(5) Which of the followings is most likely to lead to a loss of gene function? (A) A missense mutation in the open reading frame (B) A frameshift mutation in the coding region (C) A change from a T to C in the promoter region (D) A change from a TAA codon to a TAG codon in the coding region (E) A sequence change in the 3' untranslated region

1. ATP is an important molecule in metabolism because (A) It is readily obtained from an organism’s environment (B) It is extremely stable (C) It has high‐energy phosphate bonds (D) It contains valuable nutrients

2. The prokaryotic translation start with ______ initiation codon. (A) UAG (B) AUG (C) AUC (D) GUA

3. ______is a DNA sequence that controls transcription of structural genes (A) Regulatory site (B) Promoter site (C) Repressor site (D) Operator site

4. ______is binding site of RNA polymerase on sequence of DNA. (A) Promoter (B) Operator (C) Binding site (D) Regulator

5. Activation of amino acid is carried out by ______ enzyme (A) DNA Polymerase (B) Amino acyle tRNA synthatase (C) peptidyl transferase (D) both B and C

6.______ is the first amino acid in proteins of bacteria. (A) alanine (B) N formyl methionine (C) glycine (D) leucine.

7. ______enzyme relaxes the supercolling of double stranded DNA molecule (A) DNA Polymerase (B) DNA helicase (C) DNA Ligase (D) Topoisoemerase

8. ______ react with bases that contain amino group for mutation. (A) Nitrous Acid (B) Nitric Acid (C) Acetic Acid (D) Ammonium Nitrate

9. The sequence of mRNA transcribed & translated is known as (A) non coding sequence (B) mid sequence (C) intron (D) exon

10. ______ is due to less stringent pairing of third base of codon with anticodon. (A) Degeneracy (B) Mutation (C) Wobbling (D) Universality.

11. The nuclear DNA‐protein complex is called ______ (A) chromosome (B) chromatin (C) nucleoprotein (D) histone

12. The most conserved histones are______ (A) H1 & H2A (B) H2A & H2B (C) H3 & H4 (D) H1 & H4.

申論題 (13)

(1) DNA replication, transcription, and translation

(2) RNA splicing and spliceosome

(3) Recombinant DNA and protein overexpression

(4) Genetically modified food

(5) DNA promoters and terminators

(1) Please describe RISC assembly pathway for RNA degradation. (5%)

(2) Please describe upstream open reading frames and their biological roles.

(3) Please describe the attenuation in the trp operon.

4. Please translate the following paragraph into Mandarin. Double-stranded breaks (DSBs) in DNA arise frequently. If these breaks are not repaired, the consequence to the cell is disastrous. For example, a single DSB in the Escherichia coli chromosome is lethal to a cell that lacks the ability to repair it. The major mechanism used to repair DSBs in most cells is homologous recombination. Some cell types also use a simpler mechanism, such as nonhomologous end joining (NHEJ) to heal their chromosomes.

(II) Please describe the principle and process of blue‐white screening.

(III) Please describe the principle and process polymerase chain reaction.

(IV) Please describe the process of Southern blotting.

(V) Please list the types of mutations and describe their definitions.