複選題

14. What are the enzymes that possess RNA-dependent DNA polymerase activity?
(A) Pol I
(B) Klenow fragment
(C) helicase
(D) reverse transcriptase
(E) telomerase

複選題1. If you want to use genetically engineered bacteria for the production of human growth hormone, you need the following ingredients. (A) A cDNA obtained by the reverse transcription of growth hormone mRNA (B) Endoproteinases (C) Genomic DNA of the growth hormone gene (D) A DNA sequence that codes for a bacterial ribosome-binding sequence (E) A bacterial promoter sequence

複選題2. The oncogenes of the human papillomavirus (A) Activate cyclin-dependent kinases by direct binding to the catalytic subunit (B) Bind and inactivate to the retinoblastoma (C) Bind to the host cell DNA, stimulating the transcription of antiapoptotic genes (D) Bind and inactivate to the p53 protein (E) Activate growth factor receptors in the absence of the normal ligand

複選題3. Select the sequences of the two 12-residue primers that could be used to amplify the following DNA segment by PCR. (A) ATAGCGATAGGC (B) GGCGTCGGTCAG (C) CTGACCGACGCC (D) TATCGCTATCCG (E) ATAGCGCTGACC

4. In order to compare the expression of a large number of genes in rhabdomyosarcoma cells with gene expression in normal skeletal muscle, you have isolated mRNA from the two sources by affinity chromatography on an oligi-dT column. The most common method to compare the two mRNA patterns would be (A) Expression cloning (B) In situ hybridization (C) cDNA microarrays (D) RNA interference (E) Nothern blotting

複選題5. Which statement(s) is suitable to describe antisense nucleic acids? (A) Can be used to silence a pathologic gene in patients. (B) Can be produced by an inverted gene. (C) Hybridize with DNA only but not with RNA. (D) Enhance the translation when bind to messenger RNA. (E) Can be used as probes to detect a stretch of DNA in the genome.

複選題6. Which statement(s) is suitable to describe ubiquitin? (A) An isopeptide bond forms between the C-terminus of ubiquitin and the e-amino group of the modified lysine residue. (B) Ubiquitination always leads the modified proteins to degradation. (C) ATP is required for activation of ubiquitin. (D) Multiple ubiquitin molecules can be covalently linked together to form a polyubiquitin chain on the modified protein. (E) The N-terminal amino acid of the modified protein is one determinant of its half-life.

複選題7. Which statement(s) is suitable to describe DNA replication? (A) 3' to 5' polymerase activity is used to synthesize both strands of DNA. (B) DNA replication is semiconservative. (C) A short strand of RNA is always used as the primer for DNA replication in all organisms. (D) Many specialized DNA polymerases exist to govern DNA replication in different situations. (E) Native DNA polymerases usually contain so called "proof-reading" exonuclease functions.

複選題8. Which statement(s) is suitable to describe transposons? (A) Transposons usually contain short inverted terminal repeat sequences. (B) Being named as retrotransposons if RNA intermediates are involved in the transposition. (C) Transposons can cause spread of antibiotic resistance within a bacteria population. (D) Only exist in metazoan genomes. (E) Transposase is not necessarily encoded in the transposon.

複選題9. Which of the following is the DNA binding motif generally found in DNA binding protein? (A) helix-loop-helix (B) high mobility group box(HMG-box) (C) homeodomain (D) leucine zipper (E) zinc finger

10. Which of the following are true? (A) All genetic material must contain DNA. (B) No two amino acids are specified by the same codon. (C) One function of mRNA is to recognize codons. (D) Methionine can be specified by only one codon. (E) None of above is true.

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