複選題

15. Shown below is the plasmid backbone for small hairpin RNA (shRNA) used in the National RNAi Core

(A) The plasmid carries a gene for selection of E. coli that carry it
(B) The plasmid carries features that facilitate its integration into mammalian genome
(C) The plasmid carries a gene for selection of mammalian cells that carry it
(D) shRNA can be inserted to the plasmid and can be expressed driven by the U6 promoter
(E) The plasmid itself is infectious because it contains HIV elements

複選題11. Which following statement(s) is/are correct? (A) Protein glycosylation occurs immediately after protein synthesis is complete. (B) Protein glycosylation happens during the process of protein synthesis. (C) Protein glycosylation begins in endoplasmic reticulum. (D) Protein glycosylation occurs only after the protein is transported to Golgi. (E) A given protein can have different glycosylation modifications in different types of cells.

複選題12. What following alteration(s) will let an oncogene become oncogenic? (A) gene amplification (B) protein overexpression (C) CpG island methylation (D) ubiquitination (E) loss of function on a cognate inhibitor which is specific for the protein translated from the oncogene.

13. DNA pyrosequencing is a method that reads DNA sequence based on the amount of pyrophosphate generated by the DNA polymerase. For each sequencing cycle, one single triphosphate nucleotide (A) GCTAGCT (B) GCAGCT (C) GCTAGGCCT (D) GCAGGCCT (E) CGTCCGGA

14. Which of the following statements about DNA agarose electrophoresis is TRUE? (A) DNA carries negative charges and run to anode (B) Large DNA carries more negatives and run faster than small DNA (C) Large DNA run slower because it is heavier than small DNA (D) Large DNA run slower because it faces a greater resistance (E) Low percentage agarose gel provides better resolution for small DNA

16. List in the table are DNA sequences to which 11 transcription factors bind. A sequence logo was (A) Positions 5, 7, 8, and 10 are the tallest because the nucleotides are highly conserved in those positions among all sequences (B) Position 6 is taller than position 4 because position 6 is less conserved (C) Position 3 is shorter than position 11 (D) T in position 6 has the same height as T in position 9 (E) Tin position 11 is taller than G in position 12 although they appear in both positions with an equal frequency

複選題17. Which molecules can be found in Basement membrane (BM)? (A) type 1 collagen (B) type 4 collagen (C) perlecan (D) fibronectin (E) lamin A.B.C

18. which are not required for Rec A recombination repair activities. (A) ATP (B) 5' end single strand DNA (C) 3' end single strand DNA (D) Non-homologus sequences (E)

複選題19. which molecules are glycosaminoglycans? (A) hyluronic acid (B) collagen (C) condroitin sulfate (D) heparin sulfate (E) fibronectin

複選題20. GAL4 is a transcription activator in yeast which promotes expression of a group of genes. In the upstream of genes driven by GAL4, there are one or more copies of a related 17-bp sequence known as To show that UASGAL is the GAL4 responsive element, one constructed a lacZ reporter as shown in (a) and showed that the expression of lacZ is activated in galactose media in wild-type cells but not in gal4 mutants. She also did another set of experiments, in which various mutant GAL4 molecules are are examined for their transcription activator activities. Which of the following conclusions can be drawn from her experiments? (A) N terminal aal-74 is required for GAL4 binding to UAS sequence (B) N terminal aal-74 is sufficient to bind to UAS sequence (C) C terminal aa738-881 is required for GAL4 to activate lacZ expression (D) the fusion protein composed of aal-74 and aa738-881 is sufficient to activate lacZ expression. (E) aa75-737 is not dispensable for lacZ expression

21. Cellular proteins give cells structure and perform many cellular tasks except for (A) RNA-dependent RNA polymerase (B) cytoskeleton of cells (C) ion channels (D) ribozymes (E) kinases