18. Which of the following method(s) can be used for functionally inactivating a gene without altering its sequence?
(A) dominant negative constructs.
(B) RNA interference.
(C) gene knockout.
(D) a and b.
(E) b and c.

23. Which of the following in NOT true for telomeres? I. Hairpin DNA configurations. II. Repeated sequence.  III. Template-free sequence additions. IV. The length of telomere is regulated. (A) I (B) III (C) IV (D) II and IV (E) III and IV

24. Which of the following mechanisms can be used to deal with aberrant termination in eukaryotes? I. tmRNA-mediated ribosome rescue. II. exosome-mediated degradation. III. nonsense-mediated RNA decay. IV. nonsense-associated altered splicing. (A) I, II and III (B) I, II and IV (C) I, III and IV (D) II, III and IV (E) I, II, III and IV

25. An enzyme isolated from rat liver has 192 amino acid residues and is coded by a gene with 1,440 bp. Which of the following statement is correct according to the above mentioned data? I. The enzyme is not fully synthesized. II. The enzyme is coded by 576 bases out of 1440 bases.  III. The coding sequence of enzyme contains non-coding sequence of the length 764 bases. IV. The extra size of the gene is because of the presence of untranslated region present on the gene. (A) I and II (B) II and III (C) II and IV (D) I and IV (E) III and IV

26. Which of the following reagents are components of LB medium?. I. Bacto agar. II. Bacto tryptone. III. Beef extract. IV. Sodium chloride. V. Yeast extract. (A) I, II and III (B) I, III and IV (C) II, III and IV (D) II, IV and V (E) III, IV and V

複選題1. Which of the following restrict enzymes will produce a blunt end (the cutting site is indicated with * in the recognition sequence)? (A) EcoRI (G*AATTC). (B) SacI (GAGCT*C). (C) TaqI (T*CGA). (D) Stul (AGG*CCT). (E) NotI (GC*GGCCGC).

2. Which of the following features would you NOT expect to find in heterogeneous nuclear RNA (hnRNA)? (A) intron. (B) exon. (C) polyadenylation in 3' end. (D) 5' cap structure. (E) none.

3. Which of the following is     NOT     part of RNA processing in eukaryotes? (A) addition of a poly A tail. (B) splicing of exon. (C) addition of a 5' cap. (D) reverse transcription. (E) intron removal.

4. Most of the histones are well conserved from one organism to another, which histone shows the greatest variation among tissues and species? (A) H1. (B) H2A. (C) H2B. (D) H3. (E) H4.

5. Which of the following is     NOT     required by DNA polymerase for in vitro synthesis of DNA? (A) template. (B) ATP. (C) primer. (D) dATP. (E) tRNA.

6. Which of the following values is significantly unreasonable? (A) An Okazaki fragment in eukaryotes is about 150 nucleotides long. (B) An RNA primer is 5 to 15 nucleotides long. (C) The E. coli genome is 46,392 base pairs long. (D) The human genome is about 3 billion base pairs long. (E) A DNA primer is 100 to 500 nucleotides long.

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