19. Which of the following characteristics are the principles of separation of proteins in denaturing
condition during two-dimensional (2-D) gel electrophoresis experiment?
First dimension Second dimension
(A) Hydrophobicity Subunit molecular weight
(B) Density Charge
(C) Subunit molecular weight Density
(D) amino acid composition Charge
(E) isoelectronic point (pI) Subunit molecular weight