2. Physostigmine is used in the treatment of glaucoma. Within this structure, the atom indicated by is most basic, while atom is least basic.
(A) 1 (most basic), 4 (least basic)
(B) 1 (most basic), 3 (least basic)
(C) 2 (most basic), 4 (least basic)
(D) 2 (most basic), 3 (least basic)
(E) None of the above.