試題詳解

24. Four different single-stranded plasmids, A, B, C, and D are added to a tube containing substrates for DNA replication. Oligonucleotides complementary to the plasmids A, B and C were annealed to the respective plasmids. Then, plasmid A is preloaded with DNA polymerase III holoenzyme, and plasmid B is preloaded with β-clamp. What is the order of plasmid replication?
(A) All plasmids replicate at the same time.
(B) Plasmids A and B replicate first and then plasmids C and D.
(C) Plasmid A replicates first and then plasmid C, and plasmid D is the last replicated one. Plasmid B will not be replicated.
(D) Plasmid A replicates first. Plasmids B, C and D replicate later.
(E) Plasmid A replicates first, followed by plasmids B and C (in order). Plasmid D will not be replicated.