試題詳解

26. If you receive from your collaborator a DNA plasmid with a map shown below.

Which of the following methods could help you to check if you have received a right plasmid?
(A) Run the un-cut supercoiled form plasmid DNA in an agarose gel with DNA size marker. You find that the supercoiled plasmid migrates to the position between 5kb and 6 kb bands.
(B) You digest the plasmid DNA with BgIII and run the cut plasmid DNA in an agarose gel with DNA size marker. You find that the cut plasmid migrates to the position between 5kb and 6 kb bands.
(C) You digest the plasmid DNA with EcoRI and run the cut plasmid DNA in an agarose gel with DNA size marker. You find that the cut plasmid migrates to the position between 5kb and 6 kb bands.
(D) You digest the plasmid DNA with BgIII and EcoRI. Then you run the cut plasmid DNA in an agarose gel with DNA size marker. You find 4.5kb and 738bp bands after double digestion.
(E) Sequence the plasmid using a primer specific to mRFP.