28. When doing PCR to amplify a DNA fragment using mouse genomic DNA as the template, which of the following primer pair is the best choice? Forward primers:
i. ACGTCTGCCAGGCTG, 15bp, GC content=67%, Tm=57℃
ii. AGCTTGGGTTAATGCCAGGC, 20bp, GC content-55%, Tm=60℃
iii. AGCCCCATTGCTATAAGGGGAG, 22bp, GC content=55%, Tm=59℃
Reverse primers:
x. CCGTGCCAGGCAGACACGATATAA, 24bp, GC content=54%,Tm=61℃
y.AAGGCGTCAAAGTCTAAAGCG, 21bp, GC content=48%, Tm=59℃
(A) iand x
(B) ii and x
(C) ii and y
(D) iii and x
(E) jii and y
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