試卷名稱:106年 - 106 國立清華大學_碩士班考試入學試題_生命科學院甲、醫學生物科技學程:分子生物學#126172
年份:106年
科目:研究所、轉學考(插大)◆分子生物學
3. You are designing an experiment to investigate the presence of promoter binding sites for holoenzymes bearing
in B. subtilis. A DNA template is available along with the two holoenzyme complexes and the in vitro transcription buffers. Which of the following is a suitable negative control for this experiment?
(A) a reaction containing all the components minus UTP
(B) a reaction containing DNA lacking the -10 region
(C) a reaction containing DNA lacking the -35 region
(D) a reaction containing DNA lacking the UP element
(E) a reaction containing a DNA lacking the two promoter regions