39. Which of the following statements is   CORRECT   for the purification of a biological molecule?
(A) The protein G is used to purify nucleic acid and lipids.
(B) Gel-filtration chromatography is a separation method for discriminating protein hydrophobicity.
(C) Alteration of pH value or ion strength cannot interrupt the protein-ligand binding in the affinity purification.
(D) The affinity chromatography uses the specific binding properties of a protein to purify the desired protein.

1. When you decide to start a genomic study with the establishment of an expressed sequence tag or EST library, the first step you need to do is to (A) isolate total DNA from the target cells and then digest DNA with a mixture of restriction enzymes (B) isolate total RNA from the target cells and then make cDNAs via reverse transcription (C) extract cellular proteins and then digest them with selected proteinases (D) cultivate the target cells and then fix them with glutaldehyde/paraformaldehyde

2. Which of the followings is a general characteristic of the life cycle of negative-strand RNA viruses? (A) Release from cells by cell lysis (B) Genome replication occurs in the nucleus (C) Virions carry an RNA-dependent RNA polymerase (D) Structure proteins are derived from polyprotein cleavage

3. Which of the followings is an important property of enveloped viruses? (A) They are environmentally labile (B) They are released from cell by cell lysis (C) They can survive the adverse conditions of the gut (D) Antibody is sufficient for immunoprotection

4. The expression of the lac operon in E. coli is: (A) Inhibited by glucose through a process called catabolite repression. (B) Inhibited by allolactose through a process called catabolite repression. (C) Activated by lactose through activation of Crp. (D) Activated by cAMP through inhibition of LacI.

5. During the log growth phase: (A) The death rate and the duplication rate of the bacteria reach balance. (B) The density of the bacterial population increases exponentially. (C) The growth rate varies along time. (D) The stringent response is activated during log growth phase to enhance growth.

6. Bacterial flagellum: (A) Is for the motility of the bacterium (B) Is for the transformation of the bacterium (C) Is for invading a host cell (D) Is for aggregating with other bacteria

7. The difference between Thymine and Uracil base structure is at (A) OH group in the sugar portion (B) Double bond difference in nucleotide (C) Presence or absence of CH3 group (D) Position of sugar attachment

8. Shine-Dalgarno sequence is responsible for bacterial (A) Transformation (B) Transcription (C) Translation (D) Transfection

9. Which codon is   NOT   a stop codon (A) UAG (B) UGA (C) UAA (D) UUU

10. Which of the following gene transfer mechanisms is mediated by bacteriophages? (A) Transduction (B) Transposition (C) Transformation (D) Conjugation

114年 - 114 國立中山大學_碩士班招生考試試題_生科系：生物化學與分子生物學#126278

110年 - 110長庚大學_碩士班招生考試_生物醫學研究所︰生化暨分子生物學#101176

109年 - 109 長庚大學_碩士班招生考試_生物醫學研究所碩士班：生化暨分子生物學#125895

108年 - 108 長庚大學_研究所碩士班招生考試_生物醫學研究所碩士班：生化暨分子生物學#125897

107年 - 107 長庚大學_研究所碩士班招生考試試題_生物醫學研究所碩士班：生化暨分子生物學#126147

103年 - 103 長庚大學_研究所碩士班(含在職專班)招生考試_生物醫學研究所碩士班：生化暨分子生物學#126190