3. A graduate student is attempting to extract plasmid DNA from E. coli. Please devise a suitable strategy for plasmid isolation, purification and validation. (7%)

4.2005年3月中國大陸制定了《反分裂國家法》，明確提出了在以下三種情況下，中華人民共和國得採取非和平方式及其他必要措施，捍衛國家主權和領土完整是哪三種情況？

日常生活中，你可能常會聽到「多做多得、敬業樂群」做人做事的勉勵話語，你或許認同，或許感到困惑，也或許有其他體會。請以「我對多做多得敬業樂群的看法」為題目，結合自己的工作經驗、生活見聞，寫下你的感受或想法。

1. Please devise a plan for amplifying a single DNA fragment from a mixture. (3%)

2. A graduate student is trying to separate protein S from other proteins in a solution. The proteins have the following properties: Please devise effective methods for getting pure protein S. (6%)

1)What is the common molecule that these 5 hormones may be derived from? (2%)

2) Please select the correct descriptions that can apply to the receptor of T4 and T3. (3%) (a) After binding to T4, the receptors of T4 dimerize (b) After binding to T4, the receptors of T4 degrade immediately (c) After binding to T4, the receptors of T4 bind to DNA (d) After binding to T4, the receptors of T4 autophosphorylate itself (e) After binding to T4, the receptors of T4 are localized to the nucleus (f) After binding to T4, the receptors of T4 remain on the plasma membrane

3) What class of hormones are Dopamine, Norepinephrine, and Epinephrine? (2%) (a) Eicosanoid (b) Peptide hormones (c) Steroid (d) Catecholamine

4 ) Many drugs used in the treatment of psychotic disorders are dopamine antagonists. Dopamine antagonists (select the correct descriptions that apply): (3%) (a) are drugs that bind to Dopamine receptors (b) do not activate Dopamine receptors (c) do activate Dopamine receptors (d) can block the actions of dopamine (e) can trigger similar actions as dopamine (f) has a greater Kd to the receptor than Dopamine

5 ) When Dopamine signals to Dopamine receptors D1/D2 complex, Phospholipase C is activated. What are the downstream second messengers of GPCR-Phospholipase C (PLC)? (2%) (a) NO and cGMP, and calcium (b) IP3, DAG, and calcium (c) protein kinase C (PKC) (d) cAMP and calcium

6) Ca2+ is an important second messenger in many signaling pathways. Please describe how the increase in intracellular Ca2+ concentration may promote the maturation and release of Insulin in beta cells. (4%)