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112年 - 國立臺灣大學_碩士班招生考試_生物化學暨分子生物學研究所:生化學#113391
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9. Abnormalities in the
catalytic activities
of key enzymes are commonly linked to diseases. Please describe (at least) three possible causes that may lead to abnormal enzyme activities (6%).
其他申論題
3) If a DNA-binding protein forms a symmetric dimer, it is likely that the recognition site in the DNA is symmetric as well.
#484615
4) In general, the DNA replicating enzyme in E. coli is DNA polymerase I.
#484616
5) Loss of the 3' to 5' exonuclease activity of DNA polymerase in E. coli should slow the rate of DNA synthesis but not affect its fidelity.
#484617
6) Pyrimidine dimmers are usually formed between two thymines in different strands.
#484618
1) serine protease (2%)
#484620
2) allosteric regulation (3%)
#484621
3) prosthetic group (2%)
#484622
4) competitive inhibitor (3%)
#484623
11. How do you experimentally determine the Vmax (maximum velocity) and KM (Michaelis constant) of an enzyme? (4%)
#484624
12. Write all you know about a membrane bilayer. What is it for? What is it made of? What are its chemical constituents? What properties does it have? What are the methods to study its properties? What are its functions? How do its constituents work for its functions? (8%)
#484625