複選題

12. Which of the following statements regarding the "Eukaryotic Genome Organization" are CORRECT?
(A) In humans, around 30% of the entire genome actually encodes proteins.
(B) Non-coding DNA accounts for the majority of the DNA in higher animals and plants.
(C) About 50% of the human genome is repeated sequences (or repetitive sequences).
(D) Expressed genes are generally found within euchromatin.
(E)Genes for ribosomal RNA are usually found in multiple copies.

複選題8. Some small molecules that carry signals inside living cells are called second messengers. Which of the followings are second messenger(s)? (A) Calcium (Ca2+) (B) Cyclic GMP (cGMP) (C) Nitric oxide (D) Potassium (K+) (E) Cyclic AMP (CAMP)

複選題9. Which of the following statements regarding "Glycosylation" is CORRECT? (A) The sugar-modified molecules, called glycoproteins and glycolipids, constitute the majority of cell surface and extracellular proteins and lipids. (B) Golgi apparatus plays an important role in the glycosylation of proteins and lipids. (C) The N-linked sugar chains are added to asparagine residues of the protein in the ER. (D) The O-linked sugar chains are added to the hydroxyl group of tyrosine residues of a proteoglycan core protein. (E) Glycosyltransferases add specific sugar residues to glycans, while glycosidases remove specific sugar residues.

複選題10. Which of the following statements regarding "Transcription of RNA" are CORRECT? (A) RNA polymerase II synthesizes mRNA and several snRNAs involved in RNA splicing in the nucleoplasm. (B) Initiation of transcription requires RNA polymerase loading onto the chromosome at the promoter of a gene or operon. (C) Eukaryotic RNA polymerase I, II and III promoter sequences are all situated upstream of the transcription start site. (D) In addition to RNA polymerases, RNA transcription in eukaryotes also requires multiple initiation factors termed general transcription factors (GTFs). (E)The end of a gene is marked by a terminator sequence that forms a hairpin structure in the RNA.

11. The 5' End is potentially lost in replication of linear eukaryotic chromosomal DNA. Which molecules or structures are required to prevent the shortening of the 5'end of DNA? (A) DNA gyrase (B) Telomere (C) Telomerase (D) DNA polymerase (E)Helicase

13. Chromatin states created by histone modifications can be stably inherited through many rounds of cell division. The posttranslational modifications of histones include the following reactions EXCEPT FOR (A) Acetylation (B) Phosphorylation (C) Methylation (D) Ubiquitination (E) Glycosylation

14. When a change in the DNA base sequence that alters a codon so that one amino acid in a protein is replaced with a different amino acid. We call this mutation as (A) Nonsense mutation (B) Missense mutation (C) Synonymous mutation (D) Silent mutation. (E) Insertional mutation

15. Supercoiling is necessary for packaging of bacterial DNA. The enzymes that insert or remove supercoils are named: (A) Topoisomerases (B) Polymerases (C) Ligases (D) Helicases (E) Endonuclease

複選題16. RNA polymerases synthesize a new strand of nucleic acid that is complementary to one of the chromosomal DNA strands. Which of the following statements regarding RNA POLYMERASE is CORRECT? (A) RNA polymerases synthesize a strand of ribonucleotides. (B) Like DNA polymerase, RNA polymerases initiates transcription with a primer. (C) Unlike replication, the newly transcribed sequences do not remain base-paired with the template but are displaced after reaching a length of about 10 nucleotides. (D) Most eukaryotes have three different RNA polymerases, including RNA polymerase I, II, and III. (E) RNA Polymerase II is responsible for transcription of mRNA.

17. Which cytoskeleton filament supports slender protrusions of plasma membrane called microvilli or filopodia? (A) Myosin (B) Intermediate filament (C) Actin (D) Microtubule (E) Dynamin

複選題18. Which of the following statements regarding molecular biology techniques are CORRECT? (A) Southern blotting for detection of specific RNA sequences (B) Northern blotting for detection of specific DNA sequences (C) Western blotting for detection of protein expression levels (D) PCR for amplification of specific DNA sequences (E) Chromatin immunoprecipitation for detection of specific proteins that are associated with specific genomic regions