複選題

30. Which of the following statements is/are TRUE?
(A) Restriction endonucleases can cut DNA and RNA.
(B) It is common to use ddNTPs (dideoxyribonucleoside triphosphates) in DNA sequencing.
(C) Enzymes that seal nicks in DNA are called ligases.
(D) In recombinant DNA technology, a YAC is an enzyme isolated from a large South American four-legged mammal.
(E)The "c" in cDNA stands for Circular.

複選題26. Which of the following amino acids is/are a target for phosphorylation by a protein kinase? (A) Ala (B) Tyr (C) Arg (D) Thr (E)Asp

複選題27. Which of the following statements about eukaryotic translation is/are FALSE? (A) All polypeptides synthesized by cytosolic ribosomes begin with a Met residue, not an fMet. (B) A special initiator tRNA is utilized for initiation by cytosolic ribomes. (C) Polypeptides synthesized by mitochondrial ribosomes begin with fMet. (D) The 40S ribosomal subunit binds the mRNA before any initiation factors. (E)The eIF2a factor binds to the eukaryotic internal ribosome entry site (IRES).

複選題28. Which of the following is/are NOT an example of a regulatory DNA site? (A) Enhancer (B) Insulator (C) Repressor (D) Operator (E)Activator

複選題29. Below is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Which of the following statements is/are FALSE?(A) If a recombinant plasmid were obtained inserting DNA into the BamHI site, the recombinant plasmid would be resistant to the antibiotic tetracycline. (B) If a recombinant plasmid were obtained by inserting DNA into the EcoRV site, the plasmid will be resistant to ampicillin. (C) If the region between Ndel and PstI were deleted, the resulting plasmid would work well for cloning. (D) If a recombinant plasmid was obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline. (E) If the plasmid were completely digested with the three restriction enzymes BamHI, Ndel and PstI, the resulting fragments would be 1920, 1312, 754, and 375 base pairs long.

31. Which of the following is/are FALSE about the E. coli Lac operon? (A) It is polycistronic. (B) It is an example of negative control. (C) The presence of lactose acts as an inducer. (D) The repressor binds to the operator. (E)The mRNA for the repressor binds to the operator.

複選題32. Which of the following is/are required for transcription in E. coli? (A) Sigma factor (B) RNA polymerase (C) TFIID (D) DNA template (E)ATP

複選題33. Which of the following statements about the genetic code is/are CORRECT? (A) Bacterial mRNA of 800 nucleotides could code for a 300 amino acid protein. (B) There are at least three codons for each amino acid. (C) A new codon begins every three nucleotides. (D) The fourth codon establishes the reading frame. (E)The genetic code is degenerate because one particular amino acid can be encoded by more than one codon.

34. The site-specific recombinases are similar to topoisomerases in what way? (A) They introduce supercoils. (B) They require ATP hydrolysis for their activity. (C) They promote phosphoryl group transfers in which the net change in free energy is close to zero. (D) They also assist in compaction of the DNA. (E)They mediate strand invasion reactions.

複選題35. DNA sequence rearrangements are involved in the following processes? (A) immunoglobulin gene expression in mammals. (B) transposition of bacteriophage Mu. (C) mating-type switching in yeast. (D) antigen switching in trypanosomes. (E)intron splicing in ciliates.

複選題36. Which of the following base pairs is/are a Wobble base pair? (A) G-U (B) I-C (C) I-A (D) G-C (E)A-U