試題詳解

10. Although there are numerous kinds of yeast shuttle vectors, those used currently can be broadly classified in either of following three types: integrative vectors, YIp; autonomously replicating high copy-number vectors, YEp; or autonomously replicating low copy-number vectors, YCp. Scott wants to insert a DNA fragment into the yeast chromosome and he has chosen YIp as the shuttle plasmid. Which of the following sequence components are in this plasmid?
(A) E. coli genes or segments ori, bla or tet for amplification the plasmid in E. coli
(B) Yeast nutrition selectable markers (ex. URA3, HIS3, LEU2, TRP1 or LYS2)
(C) Yeast 2µ(2-micron) plasmid sequence
(D) Yeast autonomous replication sequences (ex. ARSI, ARS2 or ARS3)
(E) Yeast chromosomal centromere sequences (ex. CEN3, CEN4 or CENII)