複選題

20. GAL4 is a transcription activator in yeast which promotes expression of a group of genes. In the upstream of genes driven by GAL4, there are one or more copies of a related 17-bp sequence known as

To show that UASGAL is the GAL4 responsive element, one constructed a lacZ reporter as shown in (a) and showed that the expression of lacZ is activated in galactose media in wild-type cells but not in gal4 mutants. She also did another set of experiments, in which various mutant GAL4 molecules are are examined for their transcription activator activities. Which of the following conclusions can be drawn from her experiments?
(A) N terminal aal-74 is required for GAL4 binding to UAS sequence
(B) N terminal aal-74 is sufficient to bind to UAS sequence
(C) C terminal aa738-881 is required for GAL4 to activate lacZ expression
(D) the fusion protein composed of aal-74 and aa738-881 is sufficient to activate lacZ expression.
(E) aa75-737 is not dispensable for lacZ expression

16. List in the table are DNA sequences to which 11 transcription factors bind. A sequence logo was (A) Positions 5, 7, 8, and 10 are the tallest because the nucleotides are highly conserved in those positions among all sequences (B) Position 6 is taller than position 4 because position 6 is less conserved (C) Position 3 is shorter than position 11 (D) T in position 6 has the same height as T in position 9 (E) Tin position 11 is taller than G in position 12 although they appear in both positions with an equal frequency

複選題17. Which molecules can be found in Basement membrane (BM)? (A) type 1 collagen (B) type 4 collagen (C) perlecan (D) fibronectin (E) lamin A.B.C

18. which are not required for Rec A recombination repair activities. (A) ATP (B) 5' end single strand DNA (C) 3' end single strand DNA (D) Non-homologus sequences (E)

複選題19. which molecules are glycosaminoglycans? (A) hyluronic acid (B) collagen (C) condroitin sulfate (D) heparin sulfate (E) fibronectin

21. Cellular proteins give cells structure and perform many cellular tasks except for (A) RNA-dependent RNA polymerase (B) cytoskeleton of cells (C) ion channels (D) ribozymes (E) kinases

複選題22. Which of the following statement are correct (A) DNA severs as a template for DNA synthesis (B) RNA severs as a template for protein synthesis (C) DNA serves as a template for miRNA synthesis (D) DNA serves as a template for mRNA synthesis (E) protein serves as a template for RNA synthesis

複選題23. The fluidity of lipid membranes in cells on depends on (A) temperature (B) the amount of unsaturated fatty acyl chain (C) the length of the fatty acid chain (D) the amount of cholesterol (E) the surface area of lipid membranes

複選題24. Proteins may non-covalently interact with small molecules such as GTP, , etc. Which of the following statement is/are NOT correct? (A) after first oxygen binding, hemoglobulin adapts a new conformation and could interact the second oxygen molecule better (B) neurotransmitter glutamate triggers calcium influx by non-covalently binding to calcium channel on the post-synaptic membrane. (C) facilitates the hemophilic interaction between E-cadherin (D) binding to either GTP or GDP activates small G protein, such as Ras. (E) when cellular ATP level is high, excessive ATP non-covalently binds to and inhibits pyruvate kinase, which mediates glycolysis and ATP production

複選題25. Proteins could be covalently modified by chemicals and small peptides, such as (A) phosphorus (B) ubiquitin (C) methyl group (D) acetyl group (E) Sumo

26. If you receive from your collaborator a DNA plasmid with a map shown below. Which of the following methods could help you to check if you have received a right plasmid?(A) Run the un-cut supercoiled form plasmid DNA in an agarose gel with DNA size marker. You find that the supercoiled plasmid migrates to the position between 5kb and 6 kb bands. (B) You digest the plasmid DNA with BgIII and run the cut plasmid DNA in an agarose gel with DNA size marker. You find that the cut plasmid migrates to the position between 5kb and 6 kb bands. (C) You digest the plasmid DNA with EcoRI and run the cut plasmid DNA in an agarose gel with DNA size marker. You find that the cut plasmid migrates to the position between 5kb and 6 kb bands. (D) You digest the plasmid DNA with BgIII and EcoRI. Then you run the cut plasmid DNA in an agarose gel with DNA size marker. You find 4.5kb and 738bp bands after double digestion. (E) Sequence the plasmid using a primer specific to mRFP.