試題詳解

33. Which of the following approaches may help Michael determine the sequence of protein Q movements between the compartments X, Y and Z?
(A) Sit down in front of the microscope, and follow the movements of GFP particles in live, unfixed cultured cells in real time.
(B) Add another ligand that competes for the same receptor with protein Q, and measure the difference of GFP signals between compartments X, Y and Z.
(C) Add GFP-protein Q to the medium at 4°C. Raise the culture temperature briefly to 37°C to enable endocytosis to occur, and then go back to 4°C again. After this treatment, do the immunostaining-GFP colocalization experiment at different time points.
(D) Perform immunoEM to determine the exact localization of protein Q in X, Y or Z.
(E) Purify individual intracellular compartments and determine the level of protein Q.