試題詳解

35. Finally Michael worked out the order of protein Q trafficking as X -＞ Y -＞ Z. At the same time, Lisa
(A) Sequencing the genomic loci of all the 50 genes is the best way to identify the mutation.
(B) Since more than 50 genes are likely to be candidates for the locus harboring this mutation, it is of little help to do traditional genetic mapping.
(C) Lisa should perform 2-D gel electrophoresis to identify the mutant protein band. Remove the band from the gel and send it to the mass spectrometry - that is the simplest way to identify the mutation.
(D) Do complementation test with individual mutant strains of these 50 genes. A failure to complement will indicate that Lisa's mutation is allelic (i.e., in the same locus) to this mutant.
(E) Lisa's mutant could be a dominant, gain-of-function mutation or a recessive loss-of-function mutation.