31. Which method can determine cells at the S phase?
(A) PARP1 immunoblotting
(B) Cyclin D immunoblotting
(C) CFSE staining
(D) BrdU incorporation
(E) Cohesion cleavage

27. Which of the following statements regarding G protein or G protein-mediated signal transduction is incotrect?(1) Epinephrine binding to the beta-adrenergic receptor causes an activation of adenylyI cyclase through the activation of a stimulatory G protein.(2) Gi alpha submit of the trimeric G proteins can inhibit adenylyl cyclase(3) Gq alpha submit of the trimeric G proteins can stimulate Pl-phospholipase C (A) I and 2 (B) I and 3 (C) 2 and 3 (D) all of the above. (E) none of the above.

28. Which of the following statement about membrane potential and action potential propagation is correct?(1) The resting potential of a typical neuron is -30 my.(2) The resting membrane potential of animal neurons depend largely on nongated K+ channels.(3) During an action potential, opening of voltage-gated Cazt channels happens first.(4) Repolarization during the refractory period is largely due to opening of voltage-gated K+ channels. (A) I and 3 (B) 2 and 4 (C) 1,2 and 4 (D) 1, 3 and 4 (E) 2. 3 and 4

29. Which of the following statements regarding stem cells is incotrect? (1) Endoderm and ectodern cells are pluripotent stem cells.(2) Cord blood stem cells are multipotent stem cells.(3) Lymphoid progenitors are oligopotent stem cells. (A) 1 only (B) 2 only (C) 3 only (D) 1 and 3 (E) 2 and 3

30. Which method is NOT for detecting dead cells? (A) Trypan blue (B) Annexin V (C) Ki67 (D) Comet assay (E) Cleaved caspase-3

32. What kind of treatment does anti-PD1 or anti-PDL1 antibody work for cancer immunotherapy? (A) mRNA vaccine (B) Immune checkpoint blockade (C) CAR-T cell therapy (D) Precision medicine (E) autoimmunity

33. Which of the following repair pathway is mainly a response for repairing DNA double-stranded breaks? (A) base excision repair (B) nucleotide excision repair (C) mismatch repair (D) homologous recombination repair (E) proofreading polymerases

34. In the large-scale production of a particular human protein in insect cells, the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus. The purpose of this modification was to (A) facilitate transfer of the cDNA into the insect cells. (B) provide a promoter for the transcription of the cDNA in insect cells. (C) facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel atoms. (D) prevent degradation of the expressed protein by insect cell proteases. (E) provide the protein solubility

35. What method can be used to functionally inactivate a gene without altering its sequence? (A) gene knockout (B) RNA interference (C) dominant negative mutation (D) CRISPR-Cas9 genome editing (E) only B and C

36. Which of the following changes is more likely to further confine a membrane protein within a corral established by the cortical cytoskeletal network? (A) Binding to an extracellular ligand (B) Binding of the protein to other transmembrane proteins (C) Increased temperature (D) Protease cleavage of the cytosolic domain of the protein (E) Protease cleavage of the extracellular domain of the protein

37. Which of the following is correct regarding both Cas9 and EcoRI?(1) They are both endonucleases.(2) They both create double-strand breaks in DNA.(3) They both recognize their target sequences with the help of guide RNAs.(4) They are both part of bacterial defense mechanisms against foreign DNA.(5) They are both greatly useful in manipulating DNA and studying gene expression. (A) 1 only (B) 1,2 and 5 (C) 2,3 and 5 (D) 1, 2, 4 and 5 (E) All