阿摩線上測驗 登入

110年 - 110 國立臺灣大學_碩士班招生考試_生化科學研究所:細胞與分子生物學#102078

科目：研究所、轉學考(插大)◆細胞與分子生物學 | 年份：110年 | 選擇題數：40 | 申論題數：0

試卷資訊

所屬科目：研究所、轉學考(插大)◆細胞與分子生物學

選擇題 (40)

1. All are correct about enhancer sequences in eukaryotic cells except: (A) they can oce cur cither upstream or downstream of the gene. (B) they can be in either oricntation (bi-directional). (C) enhancer function is dependent on recognition by a specific transcription factor. (D) specific tran inscription factor binding at an enhancer stimulates transcription by interacting with RNA polymerase II. (E) All are correct.
2. Amino-acyl-tRNA synthctases catalyze the reaction of a: (A) specific amino acid attachment to the 3'-OH at the 3'-CCA of a specific tRN( A ) (B) specific amino acid attachment to the 5'-OH at the 5'-CCA of a specific tRN( A ) (C) specific tRNA with ATP to form a so called "charged tRNA" that interacts with a specific site on mRN( A ) (D) All of the above. (E) None of the above.
3. Acidification of the stomach uses all of the following types of transport EXCEPT: (A) Cl- ion channels (B) H+/K+ ATPase (C) antiporters (D) symporters (E) All of above are used by stomach parietal cells.
4. Which of the following component(s) is NOT required for the secretory pathway using cotranslational translocation machinery? (A) ribosome (B) signal recognition particle (SRP) (C) ATPase (D) translocon (E) All of above are used in the secretory pathway.
5. Which of the following pathway(s) is NOT the fate of internalized proteins after endocytosis? (A) Internalized proteins move to the lysosome for protein degtadation. (B) Internalized proteins transport to Golgi through retrograde transport (C) Internalized proteins recycle back to the plasma membrane (D) Internalized proteins associate with ribosome for newly protein synthesis (E) All of above are correct.
6. Which part of the ribosome is responsible for peptidyI transfer? (A) 18S (B) 5S (C) 23S (D) 2.8S (E) 16S

7. Given a gene sequence below, please predict the transcript(s)?
61663ad2adafc.jpg (A) S'-CGGAAC (B) 5'-AAACCG (C) 5'-AAUCCG (D) S'-UGGAAU (E) 5'-AUUCCG
8. The following methods can separate proteins based on their mass except? (A)centrifugation (B) ion exchange chromatography (C) SDS polyacrylamide gel electrophoresis (D) gel filtration chromatography (E) native polyacrylamide gel electrophoresis
9. A Holiday junction structure is a(n) (A) intermediate in genetic recombination (B) double-stranded DNA break (C) collapsed replication fork (D) thymine-thymine dimmer (E) all of the above
10. A mutation that changes a cysteine codon to a tryptophan codon is called a (A) missense mutation. (B) nonsense mutation. (C) frameshift mutation. (D) silent mutation. (E) Indel delction.

11. What do all β-barrel transmembrane proteins bave in common? (A) The structural tigidity compared to a-helical transmembrane proteins. (B) The number of negative peaks in their bydropathy plots. (C) The diameter of the barrel. (D) The number of B strands. (E) The general function, i.e. membrane transport.
12. The two monolayers of the plasma membtane in a human red blood celt : (A) both contain gangliosides. (B) both contain glycolipids. (C) have the same abundance of phosphatidylinositol. (D) exchange phospholipids only through spontaneous flip-flops. (E) have different overall elcctrical charges, with negatively charged phospholipids (c.g. phosphatidylserine) normally enriched in the inner monolayer.
13. The permeability of a protein-free lipid bilayer to various molecules depends on their properties. Sort the following in ordet of low to high permeability from lett to right. 
(1) O2 (2) ATP (3) RNA oligonucleotide (5) Glucose (6) Urea (A) (3),(2),(5),(4),(1),(6) (B)(2),(3),(6),(4).(5).(1) (C)(3),(4),(2).(5),(1),(6) (D)(3),(2).(4),(5),(6),(1) (E)(2),(3),(4).(6),(5),(1) 
14. Which one is responsible for the progression of GI to S phase? (A) Unphosphorylated Rb (B) Hypophosphorylated Rb (C) Hyperphosphorylated Rb (D) Phosphorylated p53 (E) Cyclin B
15. Which one plays the "sliding clamp" in the DNA replication? (A) Cyclin A (B) Cyclin B (C) Cyelin D (D) p53 (E) PCNA
16. Post-translational modifications modulate a protein activity. To study the functional effect of a protein phosphoprylated at Ser/Thr, we generate a non-phosphorylatable mutant by changing to Ala. What amino acids are used for creating a phosphomimetic mutant? (A) Asn and Gin (B) Lys and Arg (C) Leu and Ile (D) Asp and Glu (E) Tyr and Phe
17. Which one does not involve in splicing of introns in nuclear mRNA primary transcripts? (A) RNA helicase (B) ATP (C) CTD (C-terminal domain) of RNA polymerase I large subunit (D) snRNPs (E) endonucleases
18. How are most voltage-gated channels inactivated? (A) by closing degrading channel proteins (B) by ligand binding (C) by changes in membrane potential (D) through interaction with other membrane proteins (E) by closing spontancously soon after opening
19. Which of the following statements regarding hotmone and cellular signal transduction is correct?
(1) Amino acid and protein hormones usually act by binding to a receptor on the cell surface.
(2) A second messenger is a substance that binding to its receptor on the cell surface.
(3) cATP is an intracellular second messenger. (A) (1) (B) (2) (C) (3) (D) (1) and (3) (E) (2) and (3)
20. Which of the following shows the correct order of potency for stem cells? (A) Totipotent ＞ Multipotent ＞ Pluripotent (B) Totipotent ＞ Pluripotent ＞ Multipotent (C) Pluripotent ＞ Totipotent ＞ Multipotent (D) Multipotent ＞ Totipotent ＞ Pluripotent (E) None is correct
21. Treatment of the RNA polymerase/DNA complex with DNase in vitro is a DNA footprinting technique used to: (A) identify the termination sequence for transcription. (B) locate the start site for transcription. (C) Locate the promoter site. (D) identify the position of enbancer sequences. (E) None of the above.
22. The heptapeptide repcat in the carboxy terminal domain (CTD) in the RPB1 subunit of RNA polymerase II is phosphorylated to________. (A) convert to an elongation complex (B) bind to DNA (C) form a holo-RNA polymerase II (D) initiate transeription (E) activate termination
23. Heat shock element (HSE) is a(n)__________found in the________ region of genes whose transcription is activated in response to________.(A) silencer； enhancer； cold (B) response element； promoter, elevated temperature (C) promoter； enhancer； elevated temperature (D) enhancer； response etement: clevated temperature (E) silencer； promoter； cold
24. Which of the following statenent(s) is correct regarding to the membrane transport?
(1) If a transported molecule carries a net charge, its movement may be influenced by the electrochemical gradient.
 (2) All transport proteins, i.e. transporters, are transmembrane proteins containing multiple membrane-spanning segments.
 (3) Active transport requires ATPase to utilize ATP hydrolysis to move ions or snall molecules across the membrane from higher concentration to lower concentration. (A) 1 and 3 (B) 2 and 3 (C) 3 only (D) 1 and 2 (E) All of above are correct.
25. Which of the following statement(s) is cotrect regarding to protein endocytosis or cxocytosis?
 (1) Receptor-mediated endocytosis of ligands generally occurs through clathrin/AP2-coated vesicles.
(2) SARS-CoV-2 appears to infect cells by first binding viral spike proteins with host protein receptors. The virus is then endocytosed into the host cells.
(3) Neurotransmitters are typically relcased from synaptic vesicles into the synaptic cleft via exocytosis (A) 1 and 2 (B) 2 and 3 (C) I and 3 (D) 3 only (E) All of above are correct.
26. Which statement is correct? (A) RNA polymerase I transcribes mRNA.(B) RNA polymerase II transcribes 288 rRNA. (C) RNA polymerase III transcribes mRNA. (D) All of the above. (E) None of the above.
27. Regarding transcription factor II H (TFIIH), which statement is correct? (A) TFIH is a multimeric protein containing 11 subunits. (B) TFIIH exhibits helicase activity using GTP as an energy source. (C) TFIH phosphorylates the CTD of Polymerase II. (D) TFIIH possesses the catalytic domain of the peptidyl transfer. (E) All of the above.
28. Regarding transfer RNA (tRNA), which statement is cortect? (A) tRNA has a universal 5' -CAA-3* tail. (B) tRNA's acceptor stem maintains the anticodon region. (C) tRNA's D stem has two 8-oxoguanines. (D) tRNA has to be charged before transcription. (E) None of the above.
29. What method can be used to functionally inactivate a gene without altering its sequence? (A) gene knockout (B) RNA interference (C) dominant negative mutation (D) CRISPR/Cas9 (E) B and C
30. Which of the following is a feature that defines a cell dying via apoptosis? (A) the nucleus condenses and then fragments. (B) small membrane bodies are released and then engulfed by other cells. (C) the cell shrinks. (D) Fas ligand can trigger apoptosis (E) all of the above
31. Which of the following statements regarding transmembrane proteins is correct?
 (1) typicaily exposed only to one side of the membrane.
(2) released from the membrane by a gentle extraction procedure such as sait treatment.
(3) often further attached to the membrane via a GPI anchor.
(4) sometimes covalently attached to a fatty acid chain that inserts into the membrane.
(5) cannot contain B sheets in the part of their structure that interacts with the membrane interior. (A) (1) and (2) (B)(3) only (C) (4) only (D) (3) and (4) (E) (4) and (5)
32. Which of the following situations in a neuron results in action potentials that have extended depolarization phascs? (A) Stimulating the voltage-gated K+ channels. (B) Inhibiting the voltage-gated Na+ channels. (C) Compromising the inactivation mechanism in voltage-gated K+ channels. (D) Compromising the inactivation mechanism in voltage-gated Na+ channels. (E) None of the above.
33. What kind of DNA repair is carried out by the Crispr/Cas9 complex? (A) Homologous recombination (B) Non-homologous end-joining (C) Nuclear excision repair (D) Mismatch repair (E) Base excision repair
34. Fc receptors recognize : (A) Viral antigen (B) Variable region of antibody (C) Constant tegion of antibody (D) MHC class I (E) MHC class II
35. Which one is NOT the function of histocompatibility complex? (A) Routinely displays the degraded cellular proteins (B) Prevents auto-immune response. (C) Presents pathogen-derived peptides to immune cells. (D) Recruits NK cells (E) MHC class I presents epitopes to CD8+ cytotoxic T cell.
36. Which of the following is not known to be involved in transcription initiation by cukaryotic RNA polymerase II (Pol II)? (A) DNA helicase (B) Pol II binding to TATA box (C) Chromatin remodeling (D) The transcription factors binding to enhancers (E) Protein phosphorylation and acetylation
37. Which one of the following statements about mitochondrial DNA is incorrect? (A) Mutations in mitochondrial DNA will cause genetic diseases. (B) The mammalian milochondria DNA is wrapped by histone-like proteins. (C) Mitochondrial genetic codes differ from standard nuclear codes. (D) The products of mitochondrial genes are not exported to cytosol. (E) The mitochondria of a developing embryo are derived from the mother's egg.
38. A microRNA (miRNA) is a small non-coding RNA, which can down-regulate mRNA expression levels. Please choose the correct statement about animal miRNA.(A) A miRNA can re ecognize many mRNAs. (B) It is 10-20 nucleotides in length single-stranded RNA. (C) It is perfectly complemnentary to its mRNA targets. (D) It is a ribonuclease. (E) It most binds to the coding region of mRNA.
39. Which of the following statements regarding stem cells is correct?
 (1) Induced pluripotent stem cells can differentiate into any of the 3 germ layer cells.
 (2) Cord blood stem cells are multipotent.
 (3) Hypoxic condition in stem cell niches is necessary for maintaining sten cells in an undifferentiated state. (A) (1) (B) (2) (C) (3) (D) (1) and (3) (E) All of above
40. Which of the following statements regarding Transposable elements (TEs) is correct?
 (1) TEs are DNA sequences that have the ability to change their position within a genome.
 (2) TEs are randomly distributed in the genome.
 (3) Transposable elements can be dividcd into two major classes based on whether they use DNA or RNA as intermediate for integration into genome. (A) (I) only (B) (2) only (C) (3) only (D) (2) and (3) (E) (1) and (3)

申論題 (0)