試題詳解

34. Somehow Michael was able to solve his problem, and now he had evidence that protein Q first entered
(A) Use cultured fibroblasts that are deficient for Y, and see whether protein Q accumulates in compartment X or compartment Z. The first will indicates protein Q trafficking as X -＞ Z -＞ Y, while the latter will suggest X -＞ Y -＞ Z.
(B) Same approach as A, but accumulation in compartment X indicates the order of transport as X -＞ Y -＞ Z, whereas accumulation in compartment Z indicates the order as X -＞ Z -＞ Y.
(C) Use RNAi to interfere with the functions or biogenesis of compartments X, Y or Z, and see in which compartment is protein Q trafficking blocked.
(D) Collect and homogenize the cells at different time points, and purify compartment Y or Z by density gradient centrifugation. Test whether protein Q exists in the fraction containing Y or Z.
(E) Use a radioisotope-labeled protein Q instead of GFP, and measure the radioactivity within compartment Y or compartment Z under light microscopy.